Optimization of Virus Yield as a Strategy to Increase the Efficiency of Rabies Vaccine by BHK-21 Cells in a Bioreactor

Background: rabies is a usually fatal viral zoonotic and preventable disease. the efficacy and safety of animal rabies vaccination made in permanent bhk-21 cell culture have been proven over a long period of use. by increasing the yield of cells and viruses, the efficacy of the vaccine can be increased. objectives: the objective of this study was to optimize and maximize the output of a rabies vaccine made on bhk cells in a bioreactor. methods: this study examined the impacts of independent parameters, such as ph, temperature, cell density, and dissolved oxygen (do),onrabies virus strain pv-paris yield for a central composite design. toachieve high viral production, this study used the central composite approach to optimize cell development. results: the findings showed that bhk-21 cells were grown under the ideal conditions of ph7.21, temperature 35.05ºc, 68.75% fordo, and 2.3010^6 cell/ml of cell density to produce high titers of rabies virus (4.710^7 plaque-forming unit [pfu]/ml). high correlation coefficients (0.927) validated that the predicted model was well-fitted with the data, and the statistical analysis of the collected data indicated that the experimental data and predicted model were well-matched. the accuracy of this model’s predictions was correlated with values of adjusted r-squared (r² adj) and predicted r-squared (r² pred). conclusions: these upgrades lead to a more reliable and economical procedure that makes industrialization and commercialization easier.