evaluation of recombinant srs3 antigen for diagnosis of toxoplasmosis by enzyme-linked immunosorbent assay

Background: infections by the protozoan parasite toxoplasma gondii (t. gondii) are widely prevalent in human and animals. srs3 is a member of srs antigen family (glycosylphosphatidylinositol-linked proteins), which is structurally related to the highly immunogenic surface antigen sag1. the sag family of proteins can cover the surface of t. gondii bradyzoites and tachyzoites. objectives: the aim of this study was to evaluate the recombinant srs3 protein for diagnosis of toxoplasmosis by enzyme-linked immunosorbent assay. methods: indirect immunoglobulin g (igg) enzyme-linked immunosorbent assays (elisas) with recombinant srs3 and sag1 proteins of t. gondii were developed to evaluate the diagnosis of infection in pregnant women. results: the results showed that sensitivity and specificity were respectively 84.12% and 92% for recombinant protein srs3 and 92% and 96% for rsag1. conclusions: recombinant proteins srs3 and rsag1 are promising antigens in diagnostic assays for detection of specific antibodies against t. gondii.