بررسی اثرات مهاری عصاره هیدروالکلی گیاه تیمبرا اسپیکاتا بر رده

Introduction: lung cancer is one of the m-ain causes of death among cancers worldw-ide. since the most of current treatments for cancer are inefficient and have several side effects, finding an effective and alternative treatment has a great importance. to this end, medicinal plants have gained a special significance in recent years. the aim of this study was to examine the inhibitory effects of hydro-alcoholic extract of thymbra spi-cata on the growth of lung cancer cell line sk-mes-1.materials & methods: after cultivation of sk-mes-1 cells, these cells were treated with the several doses of hydro-alcoholic extract of thymbra spicata for 24, 48, 72 hours to quantify ic50 value. after treatm-ent, mtt was performed. furthermore, to evaluate dna fragmentation, dna was extracted 24 hours after treatment and electrophoresis was done on agarose 1%. findings: the results of mtt indicated that this extract has a dose-dependent cytotoxic effect on ssk-mes-1 cells. ic50 of this extract was determined as 110 microgr-am/ml for sk-mes-1 cells. mtt results at 48 and 72 hours had a no significant diff-erence with 24 hours. also, treatment with this extract had no any effect on dna frag-mentation.discussion & conclusion: results of this study indicated that hydro-alcoholic extract of thymbra spicata could inhibit the growth of lung cancer cell sk-mes-1 through dire-ct cytotoxic effects. further studies are su-ggested to be conducted on cell growth pat-hways to determine the molecular mechan-isms of this inhibitory effect