تاثیر تمرین هوازی و اکتاپامین بر بیان ژن شاخص های تقسیم و ادغام میتوکندری در عضله ی نعلی رت های نر تغذیه شده با روغن چند بار حرارت دیده

زمینه و هدف: پویایی میتوکندری تحت تاثیر عوامل تقسیم و ادغام می‌باشد و تمرین ورزشی می‌تواند موثر بر آن باشد. هدف از انجام پژوهش حاضر، تعیین تاثیر تمرین هوازی و اکتاپامین بر بیان ژن شاخص‌های تقسیم و ادغام میتوکندری در عضله‌ی نعلی رت‌های نر تغذیه شده با روغن چند بار حرارت دیده بود.روش کار: 25 سر رت نر ویستار بطور تصادفی به 5 گروه کنترل سالم، گروه dfo ، گروه تمرین هوازی + dfo، گروه اکتاپامین + dfo و گروه تمرین هوازی + اکتاپامین + dfo تقسیم شدند. پروتکل تمرین هوازی شامل 4 هفته تمرین هوازی، 5 جلسه در هفته به مدت 20 دقیقه دویدن بر روی تریدمیل بود. تزریق درون صفاقی اکتاپامین و گاواژ روغن حرارت دیده، به ترتیب پنج بار در هفته و هر روز انجام شد. یافته‌ها: نتایج نشان داد، مصرف روغن حرارت دیده موجب کاهش معنی‌دار  mfn2(0/05>p)، افزایش معنی‌دار (p<0/05)drp1 و عدم تغییر معنی‌دار (p>0/05)mda در مقایسه با گروه سالم شد. اثر تعامل تمرین هوازی و اکتاپامین باعث کاهش معنی‌دار بیان ژن (p<0/05)drp1 و اختلاف غیر معنی‌دار بیان ژن mfn2 و غلظت (p>0/05)mda در مقایسه با گروه dfo شد.نتیجه‌گیری: مصرف روغن حرارت دیده، پویایی میتوکندری در عضله را مختل می‌کند. به نظر می‌رسد تعامل تمرین هوازی و اکتاپامین می‌تواند باعث بهبود پویایی میتوکندری و استرس اکسیداتیو در عضله شود.

effect of aerobic training and octopamine on the gene expression of mitochondrial fission and fusion markers in soleus muscle of male rats fed with repeated heated oil

background & aims: cooking with re heated oils result in the release of acrylamide from starch at higher doses than the recommended limits. heterocyclic amine is another toxin that is produced in this process, which is formed through the heating and cooking process form the protein substances when amino acids and creatine simultaneously react at high cooking temperatures (barbecued/fried foods). reheated oils contain oxidized monomers, dimers, polymers, free radicals, reactive oxygen species (ros), hydroperoxidases and aldehydes. these products have detrimental effects on human health and can cause harmful changes in body organs. mitochondrial dynamics and morphology have increasingly been shown to be regulated by ros. ros are central to redox homeostasisthe balance between reduction and oxidation reactions via the gain or loss of electrons. mitochondrial dynamics factors include opa1 protein (optic atrophy 1) and mitofusin 1 and 2 (mfn1 and 2), dynaminrelated protein (drp1) and fusion protein 1. deviation towards fusion optimizes mitochondrial function and is useful in maintaining longterm bioenergy capacity. conversely, deviation toward division leads to the removal of the damaged part of the mitochondria. exercise has recently been recognized as an effective way to increase mitochondrial function, and the role of exercise in improving mitochondrial damage and oxidative stress in various diseases has been reported. on the other hand, recently, attempts have been made to diminish the adverse effects of acrolein in the reheated oils through exercise training and its synergistic effects with supplementations. today, herbal supplements are considered highly effective in the reduction of oxidative stress and inflammation. octopamine supplementation has been reported to have antioxidant properties. octopamine is used as a food ingredient, as well as medicinal and dietary supplementation. octopamine is an adrenergic substance with a sympathetic function. weight loss and adrenergic actions are associated with metabolic changes. some of the main functions of octopamine are in antioxidant and antiinflammatory processes, weight loss, fat burning, and anticancer treatments. on the other hand, recent findings have indicated that exercise training could decrease oxidative stress and inflammation and improve antioxidant enzymes. meanwhile, some studies have reported that exercise training has no significant impact on inflammation and oxidative stress. the aim of present study was to investigate the effect of aerobic training and octopamine on the gene expression of mitochondrial fission and fusion markers of soleus muscle of male rats fed with repeated heated oil.methods: in an experimental study, 15 male wistar rats (age: 20 weeks, weight: 300350 g) were randomly divided into five groups, healthy control (n=3), intoxication (dfo, n=3), aerobic training + intoxication (n=3), octopamine + intoxication (n=3) and aerobic training + octopamine + intoxication (n=3). the exercise training program was conducted for four weeks at moderate intensity every other day. the intensity of exercise training in the first week reached 50% of the maximum consumed oxygen, while it reached 65% of the maximum consumed oxygen in the last week. in order for the animals to adapt to the exercise protocol, an adaptation exercise training session was implemented at the speed of nine meters per minute for 20 minutes during one week before the main exercise training program. the duration of exercise training was 20 minutes, with the intensity set at 16 meters per minute on the first day and 26 meters per minute on the last day of the intervention.