مقایسه اثر شدت‌های مختلف تمرین هوازی بر بیان فاکتورهای رونویسی e2f1 و e2f4 در بافت کبد موش‌های صحرایی نژاد ویستار

زمینه و هدف: هدف این تحقیق بررسی تاثیر سه شیوه‌ی تمرینات mit، hit، hiit بر بیان ژن های بیان ژن های  e2f1و e2f4 در بافت کبد رت های نر نژاد ویستار بود.روش کار: بدین منظور 32 سر رت نر نژاد ویستار با میانگین سنی 8 هفته و وزن 33 ± 237 گرم به صورت تصادفی به چهار گروه کنترل (co)، تمرین با شدت متوسط (mit)، تمرین پرشدت (hit)، تمرین تناوبی پرشدت (hiit) تقسیم شدند و مقادیر بیان ژن های e2f1 و e2f4 در آن­ها مورد بررسی قرار گرفت.یافته ها: نتایج نشان داد که تفاوت معنی‌داری در بیان ژن e2f1 در بافت کبد رت‌های نر ویستار میان گروه hit نسبت به گروه کنترل تفاوت معنی‌داری وجود دارد. ضمناً اختلاف معنی داری میان گروه های mit و کنترل مشاهده شد (0/003 =p). مقایسه بین گروهی با آزمون تعقیبی نشان داد که اختلاف معنی‎داری میان گروه‌های hit و گروه‌های mit، hiit و کنترل وجود ندارد (به ترتیب 0/977 =p= 0/093 ،p= 0/917،p). بررسی آزمون تعقیبی در گروه‌های تمرینی نشان داد که اختلاف معنی‌داری در بیان ژن e2f4 میان گروه‌هایmit و گروه‌های hit، hiit و کنترل وجود ندارد (به ترتیب 0/977 =p=0/202 ،p=0/721 ،p). از سویی دیگر تفاوت معنی‌داری بین گروه hiit نسبت به گروه کنترل مشاهده شد (به ترتیب 0/022=p).نتیجه‌گیری: به طور کلی نتایج نشان داد که بین ٣ شیوه mit،hit وhiit بر بیان ژن e2f1 و e2f4 در بافت کبد رت های نر نژاد ویستار تفاوت معناداری وجود ندارد. ولیکن بین گروه های تمرینی با کنترل تفوات معنی داری وجود دارد

comparison of the effect of different intensities of aerobic exercise on the expression of transcription factors e2f1 and e2f4 in liver tissue of wistar rats

background & aims: the liver is one of the main and major organs of the body, which with the help of various enzymes is of special importance in regulating hormonal activities and metabolism, during rest, training, and returning to the original state of sports activities. under normal circumstances, the liver and kidneys receive 27% and 22% of circulating blood, respectively, but as a result of heavy training, the blood flow to the liver and kidneys decreases to 5% and 3%, respectively. prolonged reduction in blood flow to the liver and kidneys may have detrimental consequences, of which fatigue observed as a result of the constant submaximal activity is part of it. the liver is also one of the most sensitive tissues targeted by traininginduced oxidative stress. sports science coaches and professionals are working to find ways to reduce the effects of strenuous training and increase the effectiveness of training programs over a limited period. the liver has different functions in the body and different roles are considered for it. metabolism, control of glycolysis, glycogenesis, and lipogenesis are the main functions of liver tissue. many factors and proteins play a role in these processes, and two factors have recently been identified called e2f1 and e2f4. methods: the present study was approved by the ethics committee of payame noor university with the code ir.pnu.rec.1398.059. in terms of purpose, it is fundamentalapplied, which was implemented experimentally. in the present study, 32 8weekold male wistar rats with an average weight of 237 ±33 g were purchased from the pasteur institute. after being transferred to the animal laboratory environment, these animals are housed in transparent polycarbonate cages in an environment with a temperature of 22 ± 1.4 °c, the humidity of 45 to 55%, four heads in each cage with free access to water and closed. foods were maintained according to a 12hour sleepwake cycle. animals were randomly divided into 5 groups: control group (co) (8 heads), moderate intensity training (mit) (8 heads), highintensity training (hit) (8 heads), and highintensity interval training (hiit) (8 heads) were divided.the mit protocol was performed in such a way that in the first week, 5 minutes of warmup, 5 minutes of cooling, and 20 minutes of the main body of the exercise, including running at 65% vo2max at a speed of 20 m/min, was added to the training time every week. in the sixth week, the training time reached 37 minutes and remained constant until the end of the eighth. also, the training speed was unchanged from the first week to the eighth week and was equal to 20 meters per minute.