effects of ceratonia siliqua l., extract-loaded nanoliposomes on a rat model of excisional wound healing

Wound healing consists of inflammatory, proliferation and remodeling phases. the objective of the current study was to investigate the effect of ceratonia siliqua l., extract-loaded liposomes on wound healing. ceratonia siliqua l., commonly called carob, is an evergreen tree that belongs to the leguminosae family widely cultivated in mediterranean countries. twenty-five healthy adult male wistar rats were randomized into five groups of five animals each: sham group with only wound creation. eucrn group: as a control group with wound creation as well as 5 g eucerin administered topically to the wound bed. nlpsm group: as a group with wound creation as well as 5 ml nanoliposome (20 mg). eucrn/nlpsm group: as a group with wound creation as well as 5 g eucerin containing 5 ml nanoliposome (20mg). treatment group: as a group with wound creation as well as 5 ml eucerin (5 g) containing nanoliposome (20 mg) loaded with hydroalcoholic extract of ceratonia siliqua l. the excisional wound model was used for biochemical, histopathological and planimetric assessments. the wound area was significantly reduced in the treatment group compared to other groups (p < 0.05). biochemical and quantitative histopathological analyses revealed a significant difference between treatment and other groups (p < 0.05). ceratonia siliqua l., extract-loaded nanoliposomes showed the potential to improve wound healing significantly. this appeared to work by angiogenesis stimulation, fibroblast proliferation, inflammation reduction, and granulation tissue formation during the initial stages of the healing process. this accelerated healing led to earlier wound area reduction of the damaged area due to the reorganization of granulation tissue and collagen fibers. topical administration of ceratonia siliqua l., extract-loaded nanoliposomes could be recommended for wound healing due to reducing wound healing acceleration.