مقایسه اثر 4 هفته تمرین هوازی و مقاومتی بر بیان ژن عضلانی فاکتور بازدارنده لوسمی در موش های صحرایی تغذیه شده با رژیم غذایی پرچرب

ﻣﻘﺪﻣﻪ: ﻓﺎﮐﺘﻮر ﺑﺎزدارﻧﺪه ﻟﻮﺳﻤﯽ ) lif( ﻣﯿﻮﮐﯿﻨﯽ اﺳﺖ ﮐﻪ ﺗﺤﺖ ﺗﺎﺛ ﯿﺮ اﺿﺎﻓﻪ وزن ﻗﺮار ﮔﺮﻓﺘﻪ و دارای اﺛﺮات اﻟﺘﻬﺎﺑﯽ و ﻣﺨﺮب اﺳﺖ. از آﻧﺠﺎﯾﯽ ﮐﻪ ورزش ﺑﺎﻋﺚ ﮐﺎﻫﺶ وزن اﺿﺎﻓ ﯽ ﻣﯽﺷﻮد، ﻫﺪف ازاﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﺄﺛﯿﺮ ﺗﻤﺮﯾﻨﺎت ﻫﻮازی و ﻣﻘﺎوﻣﺘﯽ ﺑﺮ ﺑﯿﺎن ژن ﻋﻀﻼﻧﯽ lif در ﻣﻮشﻫﺎی ﺻﺤﺮاﯾﯽ ﺗﻐﺬﯾﻪ ﺷﺪه ﺑﺎ رژﯾﻢ ﻏﺬاﯾﯽ ﭘﺮﭼﺮب ﻣﯽﺑﺎﺷﺪ. روش ﺗﺤﻘﯿﻖ: دراﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﺠﺮﺑﯽ، 32 ﻣﻮش ﺻﺤﺮاﯾﯽ ﺑﻪ ﻃﻮر ﺗﺼﺎدﻓﯽ ﺑﻪ ﭼﻬﺎر ﮔﺮوه ﮐﻨﺘﺮل ﺳﺎﻟﻢ، ﮐﻨﺘﺮل ﭼﺎق، ﭼﺎق+ ورزش ﻫﻮازی و ﭼﺎق+ ﺗﻤﺮﯾﻦ ﻣﻘﺎوﻣﺘ ﯽ ﺗﻘﺴ ﯿﻢ ﺷﺪﻧﺪ. ﺗﻤﺮﯾﻦ ﺑﻪ ﻣﺪت 4ﻫﻔﺘﻪ ﺑﺎ ﺷﺪت ﻫﻮازی ﻣﺘﻮﺳﻂ ) 50ﺗﺎ 65درﺻﺪ vo2max( اﻧﺠﺎم ﺷﺪ. ﺑﺮای ﺗﻤﺮﯾﻦ ﻣﻘﺎوﻣﺘﯽ ﻧﯿﺰ ﻣﻮش ﻫﺎ ﺑﺮای ﺑﺎﻻ رﻓﺘﻦ از ﻧﺮدﺑﺎن آﻣﻮزش داده ﺷﺪﻧﺪ )ارﺗﻔﺎع 110ﺳﺎﻧﺘﯽﻣﺘﺮ، ﺷﯿﺐ80 درﺻﺪ و ﻓﺎﺻﻠﻪ ﺑﯿﻦ ﻣﯿﻠﻪﻫﺎی ﻧﺮدﺑﺎن 2ﺳﺎﻧﺘﯽﻣﺘﺮ( ﮐﻪ ﺑﺮ اﺳﺎس ﺗﻌﯿﯿﻦ ﺣﺪاﮐﺜﺮ ﯾﮏ ﺗﮑﺮار اﺳﺖ. ﯾﮏ رژﯾﻢ ﻏﺬاﯾﯽ ﭘﺮﭼﺮب ﺑﺎ 40درﺻﺪ ﭼﺮﺑﯽ، 13 درﺻﺪ ﭘﺮوﺗﺌﯿﻦ و 47درﺻﺪ ﮐﺮﺑﻮﻫﯿﺪرات ﺗﻬﯿ ﻪ ﺷﺪ و ﺗﺎ زﻣﺎﻧﯽ ﮐﻪ ﻣﻮشﻫﺎ ﺑﻪ ﻣﺤﺪو ده ﭼﺎﻗﯽ رﺳﯿﺪﻧﺪ اداﻣﻪ ﯾ ﺎﻓﺖ. ﻧﻤﻮﻧﻪ ﺑﺎﻓﺖ از ﻋﻀﻠﻪ ﮔﻠﻮﺗﺌﻮس ﮔﺮﻓﺘﻪ ﺷﺪ. ﯾﺎﻓﺘﻪﻫﺎ: ﺑﯿﺎن lif در ﮔﺮوه ﮐﻨﺘﺮل ﭼﺎق ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل ﺳﺎﻟﻢ اﻓﺰاﯾﺶ ﻣﻌﻨﯽداری داﺷﺖ )0/039=p(. ﺑﯿﺎن lif در ﮔﺮوه ﺗﻤﺮﯾﻦ ﻣﻘﺎوﻣﺘﯽ ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل ﭼﺎق ﮐﺎﻫﺶ ﻣﻌﻨﯽداری داﺷﺖ )0/046=p(. ﺗﻔﺎوت ﻣﻌﻨﯽداری ﺑﯿﻦ ﮔﺮوه ﺗﻤﺮﯾﻦ ﻫﻮازی ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل ﭼﺎق وﺟﻮد ﻧﺪاﺷﺖ )p=0/059(. ﺗﻔﺎوت ﻣﻌﻨﯽداری ﺑﯿﻦ دو ﮔﺮوه ﺗﻤﺮﯾﻦ ﻫﻮازی و ﻣﻘﺎوﻣﺘﯽ از ﻧﻈﺮ 1-fstl وﺟﻮد داﺷﺖ )0/042= p(. ﻧﺘﯿﺠﻪﮔﯿﺮی: ﺑﻪ ﻧﻈﺮ ﻣﯽرﺳﺪ ﺗﻤﺮﯾﻦ ﻣﻘﺎوﻣﺘﯽ در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ورزش ﻫﻮازی ﺗﺄﺛﯿﺮ ﺑﯿﺸﺘﺮی ﺑﺮ ﺑﯿﺎن lif در ﻋﻀﻼت ﻧﻤﻮﻧﻪﻫﺎی ﭼﺎق دارد.

comparison of the effect of 4 weeks of aerobic and resistance training on muscle gene expression of leukemia inhibitory factor in rats fed with a high-fat diet.

purposes: leukemia inhibitory factor (lif) is a myokine that is affected by overweight and has inflammatory and destructive effects. since exercise causes excess weight loss, the aim of this study is the effect of aerobic and resistance exercises on muscle lif gene expression in rats fed a high-fat diet.materials and methods: in this experimental study, 32 rats were randomly divided into four groups: healthy control, obese control, obese + aerobic exercise, and obese + resistance exercise. the training was done for 4 weeks with moderate aerobic intensity (50-65% of vo2max). for resistance training, rats were trained to climb a ladder (height 110 cm, slope 80%, and the distance between the bars of the ladder 2 cm), based on determining the maximum of one repetition. a high-fat diet of 40% fat, 13% protein, and 47% carbohydrate was prepared and continued until the mice reached the obesity threshold. the tissue sample was taken from the gluteus muscle.results: the expression of lif in the obese control group was significantly increased compared to the healthy control group (p=0.039). the expression of lif in the resistance training group was significantly decreased compared to the obese control group (p=0.046). there was no significant difference between the aerobic exercise group and the obese control group (p=0.059). there was a significant difference between the aerobic and resistance training groups regarding fstl-1 (p=0.05).conclusion: resistance training seems to have a greater effect on lif expression in the muscles of obese samples compared to aerobic exercise.