طراحی سازه کاندید واکسن برای گونه های بیماری زای آیمریای طیور ایران با استفاده از روش های ایمونوانفورماتیک

زﻣﯿﻨﻪ و ﻫﺪف: ﮐﻮﮐﺴﯿﺪ ﯾﻮز ﻃﯿﻮر را ﮔﻮﻧﻪ ﻫﺎی ﻣﺨﺘﻠﻒ آﯾ ﻤﺮﯾﺎ اﯾﺠﺎد ﻣﯽﮐﻨﻨﺪ، اﯾﻦ اﻧﮕﻞ ﺗﮏ ﯾﺎﺧﺘﻪای ﻣﺘﻌﻠﻖ ﺑﻪ ﺷﺎﺧﮥ اﭘﯽ ﮐﻤﭙﻠﮑﺲ اﺳﺖ. اﯾﻦ ﺑﯿﻤﺎری ﯾﮑﯽ از ﺑﯿﻤﺎریﻫﺎی راﯾﺞ در ﻃﯿﻮر اﺳﺖ ﮐﻪ در ﺳﺮاﺳﺮ ﺟﻬﺎن ﺿﺮرﻫﺎی اﻗﺘﺼﺎدی ﻋﻤﺪهای ﺑﻪ ﺑﺎر ﻣﯽآورد. ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ اﯾﺠﺎد ﺿﺮر و زﯾﺎن اﻗﺘﺼﺎدی در ﺻﻨﻌﺖ ﻃﯿﻮر و ﭘﯿﺸﮕﯿﺮی از ﺑﺮوز ﻣﻘﺎوﻣﺖ ﺷﯿﻤﯿﺎﯾﯽ، ﺗﺤﻘﯿﻘﺎت ﺑﻪ ﺳﻤﺖ روﯾﮑﺮدﻫﺎی ﺟﺎﯾﮕﺰﯾﻦ ﻣﺎﻧﻨﺪ روش ﻫﺎی اﯾﻤﻮﻧﻮﻟﻮژﯾﮏ ﺳﻮق داده ﺷﺪه اﺳﺖ. ﺗﻬﯿﮥ واﮐﺴﻦ، روش ﺟﺎﯾﮕﺰﯾﻨﯽ اﺳﺖ ﮐﻪ در آن اﻧﺘﺨﺎب ﯾﮏ ﭘﺮوﺗﺌﯿﻦ ﻣﻨﺎﺳﺐ ﺑﺎ ﻗﺪرت اﻟﻘﺎی اﯾﻤﻨﯽ ﺑﺎﻻ ﯾﮏ ﭘﯿﺶ ﻧﯿﺎز ﻣﺤﺴﻮب ﻣﯽﺷﻮد. ﻋﻼوه ﺑﺮ اﯾﻦ، ﻃﺒﻖ ﻣﻄﺎﻟﻌﺎت، اﺳﺘﻔﺎده از ﺑﯿﺶ از ﯾﮏ ﭘﺮوﺗﺌﯿﻦ ﻣﯽﺗﻮاﻧﺪ اﻟﻘﺎی اﯾﻤﻨﯽ و ﺗﻮﻟﯿﺪ آﻧﺘﯽ ﺑﺎدی را ﺑﻬﺘﺮ اﻓﺰاﯾﺶ دﻫﺪ. اﻧﺘﺨﺎب اﭘﯽﺗﻮپ ﻫﺎی اﯾﻤﻮﻧﻮژﻧﯿﮏ از ﭘﺮوﺗﺌﯿﻦﻫﺎی ﻣﻨﺘﺨﺐ و ﭼﺴﺒﺎﻧﺪن آن ﻫﺎ ﺑﺎ ﯾﮏ ﭘﯿﻮﻧﺪ دﻫﻨﺪة ﻣﻨﺎﺳﺐ، ﯾﮑﯽ از روﯾﮑﺮدﻫﺎی ﺟﺪﯾﺪ در ﻃﺮاﺣﯽ واﮐﺴﻦ اﺳﺖ.ﻣﻮاد و روش ﻫﺎ: ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ اﯾﻨﮑﻪ ﻫﺮ دو ﭘﺮوﺗﺌﯿﻦ 1-imp و 1-ama آﯾﻤﺮﯾﺎ ﻗﺒﻼً ﺑﻪ ﻋﻨﻮان آﻧﺘﯽ ژنﻫﺎی اﯾﻤﻨﯽزای ﻗﻮی ﺷﻨﺎﺧﺘﻪ ﺷﺪهاﻧﺪ، در اﯾﻦ ﻣﻄﺎﻟﻌﻪ اﭘﯽﺗﻮپﻫﺎی اﯾﻤﻮﻧﻮژﻧﯿﮏ اﯾﻦ ﭘﺮوﺗﺌﯿﻦﻫﺎ ﺑﺎ روش ﻫﺎی اﯾﻤﻮﻧﻮ اﻧﻔﻮرﻣﺎﺗﯿﮏ ﭘﯿﺶ ﺑﯿﻨﯽ ﺷﺪهاﻧﺪ. در ﺑﯿﻦ اﭘﯽﺗﻮپﻫﺎی ﭘﯿﺸﻨﻬﺎد ﺷﺪه، اﭘﯽﺗﻮپ ﻫﺎﯾﯽ ﮐﻪ ﺑﺎ اﺑﺰارﻫﺎی ﺑﯿﻮاﻧﻔﻮرﻣﺎﺗﯿﮏ ﻣﺘﻌﺪد ﺗﺄﯾﯿﺪ ﺷﺪﻧﺪ، اﺳﺘﻔﺎده ﺷﺪ . ﯾﺎﻓﺘﻪ ﻫﺎ: ﺑﺎ اﺗﺼﺎل اﭘﯽﺗﻮپ ﻫﺎی اﻧﺘﺨﺎﺑﯽ و اﺗﺼﺎل آن ﻫﺎ ﺑﺎ ﻟﯿﻨﮑﺮﻫﺎی ﻣﻨﺎﺳﺐ، ﺳﺎﺧﺘﺎر ﭘﻠﯽ ﺗﻮﭘﯿﮑﯽ ﻃﺮاﺣﯽ ﺷﺪ.ﻧﺘﯿﺠﻪ ﮔﯿﺮی : ﺑﺎ اﺳﺘﻔﺎده از اﺑﺰارﻫﺎی اﯾﻤﻮﻧﻮ اﻧﻔﻮرﻣﺎﺗﯿﮏ، اﭘﯽﺗﻮپ ﻫﺎی دو ژن ama-1 و 1-imp از ﺳﻮﯾﻪ ﻫﺎی اﻧﮕﻞ آﯾﻤﺮﯾﺎ ﺗﺮﮐﯿﺐ ﺷﺪه و ﺑﻪ ﻋﻨﻮان ﮐﺎﻧﺪﯾﺪای واﮐﺴﻦ ﻗﻮی ﭘﯿﺶﺑﯿﻨﯽ ﺷﺪ.

designing a candidate vaccine structure for eimeria pathogenic species of poultry using immunoinformatics methods

background and aim: coccidiosis is caused by various species of eimeria, a protozoan parasite belonging to the phylum epicomplexa.this disease is one of the common diseases in poultry that causes major economic losses all over the world. due to the experience of economic loss in production and prevention of the disease and occurrence of chemical resistance, researches have been shifted toward alternative approaches such as immunologic methods. vaccine preparation is an alternative way in which choosing an appropriate protein with high immune induction potency is a prerequisite. in addition according to studies, using more than one protein could better enhance the immune induction and antibody production. choosing immunogenic epitopes from selected proteins and adjoining them with a suitable linker is one of the novel approaches in vaccine design.materials and methods: based on the fact that both imp-1 and ama-1 proteins of eimeria were previously recognized as potent immunogenic antigens, we predicted the immunogenic epitopes of these proteins by immunoinformatic methods. among studied epitopes, those that were met by multiple bioinformatics tools were used.results: finally, the polytopic construction was designed by assembling the selected epitopes and connecting thoseusing suitable linkers.conclusion: using immunoinformatic tools, we predicted the characteristics of two genes of eimeria strains as fused potent vaccine candidates namely, ama-1 and imp-1.