جستجوی ژنومی کوکسیلا بورنتی در نمونه‌های شیر گاو استان لرستان براساس ژن ترانسپوزونی is1111

زﻣﯿﻨﻪ و ﻫﺪف: ﭘﮋوﻫﺶ ﺣﺎﺿﺮ ﺑﺎ ﻫﺪف ﺟﺴﺘﺠﻮی ﻣﻮﻟﮑﻮﻟﯽ ﮐﻮﮐﺴﯿﻼ ﺑﻮرﻧﺘﯽ در ﻧﻤﻮﻧﻪ ﻫﺎی ﺷﯿﺮ ﺧﺎم ﺟﻤﻊ آوری ﺷﺪه از ﮔﺎوﻫﺎ در اﺳﺘﺎن ﻟﺮﺳﺘﺎن اﻧﺠﺎم ﺷﺪه اﺳﺖ. ﻣﻮاد و روش ﻫﺎ : اﯾﻦ ﭘﮋوﻫﺶ ﺑﻪ ﺻﻮرت ﻣﻘﻄﻌﯽ و ﺗﻮﺻﯿﻔﯽ اﻧﺠﺎم ﺷﺪه اﺳﺖ. ﺗﻌﺪاد ﮐﻠﯽ 200 ﻧﻤﻮﻧﻪ ﺷﯿﺮ ﺑﻪ ﻃﻮر ﺗﺼﺎدﻓﯽ از ﮔﻠﻪﻫﺎی ﮔﺎوداری ﻫﺎی ﺳﻨﺘﯽ ﻣﺘﻌﻠﻖ ﺑﻪ دو ﺷﻬﺮ )ﺧﺮمآﺑﺎد و ﭘﻠﺪﺧﺘﺮ( اﺳﺘﺎن ﻟﺮﺳﺘﺎن، ﺟﻤﻊآوری ﺷﺪ. ﻧﻤﻮﻧﻪ ﻫﺎی ﺷﯿﺮ در ﻓﺼﻞ زﻣﺴﺘﺎن ﺳﺎل 1400ﺟﻤﻊآوری ﺷﺪ، ﻫﻤﭽﻨﯿﻦ ﺳﻦ ﺣﯿﻮاﻧﺎت ﺛﺒﺖ ﺷﺪ. از ﻫﻤﮥ ﻧﻤﻮﻧﻪ ﻫﺎی ﺷﯿﺮ dna اﺳﺘﺨﺮاج ﺷﺪ. ﺳﭙﺲ ﺑﺮا ی ﺗﺸﺨﯿﺺ ﮐﻮﮐﺴﯿﻼ ﺑﻮرﻧﺘﯽ ﺑﺮ اﺳﺎس ژن ﺗﺮاﻧﺴﭙﻮزوﻧﯽ is1111از واﮐﻨﺶ nested-pcr اﺳﺘﻔﺎده ﺷﺪ. ﻫﻤﭽﻨﯿﻦ ﺟﻬﺖ آﻧﺎﻟﯿﺰ آﻣﺎری از ﻧﺮماﻓﺰار spssو روش ﮐﺎی دو ﻣﺮﺑﻊ اﺳﺘﻔﺎده ﮔﺮدﯾﺪ. isﻧﺸﺎن داد ﮐﻪ 5درﺻﺪ ) 95درﺻﺪ 0/7-55 : ciدرﺻﺪ( از ﻧﻤﻮﻧﻪ ﻫﺎی ﺷﯿﺮ ﻣﻮرد ﺑﺮرﺳ ﯽ، از ﻧﻈﺮ ﺑﺎر ﻣﯿﮑﺮو ﺑﯽ ﮐﻮﮐﺴﯿﻼ ﺑﻮرﻧﺘﯽ ﻣﺜﺒﺖ ﺑﻮدﻧﺪ. ﻣﺸﺨﺺ ﺷﺪ ﮐﻪ ﺑﯿﻦ ﺳﻦ ﮔﺎو ﺑﺎ دﻓﻊ ﮐﻮﮐﺴﯿﻼ ﺑﻮرﻧﺘﯽ از ﻃﺮﯾﻖ ﺷﯿﺮ ﮔﺎوﻫﺎی اﺳﺘﺎن ﻟﺮﺳﺘﺎن ارﺗﺒﺎط ﻣﻌﻨﯽ داری وﺟﻮد دارد )0/05>p( .ﻧﺘﯿﺠﻪ ﮔﯿﺮی : ﺑﺮ اﺳﺎس ﯾﺎﻓﺘﻪﻫﺎی اﯾﻦ ﭘﮋوﻫﺶ ﻣﯽ ﺗﻮان ﺑﻪ اﯾﻦ ﻧﺘﯿﺠﻪ رﺳﯿﺪ ﮐﻪ ﺷﯿﺮ ﮔﺎو ﻣﯽ ﺗﻮاﻧﺪ ﺑﻪ ﻋﻨﻮان ﯾﮑﯽ از ﻣﻨﺎﺑﻊ ﻣﻬﻢ در اﻧﺘﻘﺎل ﺑﺎﮐﺘﺮی ﮐﻮﮐﺴﯿﻼ ﺑﻮرﻧﺘﯽ ﺑﻪ ﻣﯿﺰﺑﺎن ﻫﺎی ﺑﻌﺪی، در ﻧﻈﺮ ﮔﺮﻓﺘﻪ ﺷﻮد. ﺑﻨﺎﺑﺮاﯾﻦ، ﺑﻪ دﻟﯿﻞ ﻣﺎﻧﺪﮔﺎری ﺑﺎﻻی ﮐﻮﮐﺴﯿﻼ ﺑﻮرﻧﺘﯽ ﺑﻪ ﻋﻠﺖ وﺟﻮد ﻓﺮم ﺷﺒﻪ اﺳﭙﻮر، ﺧﻄﺮ اﻧﺘﻘﺎل ﮐﻮﮐﺴﯿﻼ ﺑﻮرﻧﺘﯽ از ﻃﺮﯾﻖ ﺷﯿﺮ ﺧﺎم و ﻣﺤﺼﻮﻻت ﻟﺒﻨﯽ ﭘﺎﺳﺘﻮرﯾﺰه ﻧﺸﺪه، ﻧﻤﯽﺗﻮان از آن ﭼﺸﻢ ﭘﻮﺷﯽ ﮐﺮد. ﺑﻨﺎﺑﺮاﯾ ﻦ، ﺷﯿﺮ ﮔﺎو ﺑﺎﯾﺪ ﺑﻪ ﻋﻨﻮان ﻋﺎﻣﻞ ﻣﻬﻤﯽ در اﭘﯿﺪﻣﯿﻮﻟﻮژی ﺗﺐ ﮐﯿﻮ و ﺳﻼﻣﺖ ﻋﻤﻮﻣ ﯽ در ﻣﻨﻄﻘﮥ ﻣﺮﮐﺰی اﺳﺘﺎن ﻟﺮﺳﺘﺎن در ﻧﻈﺮ ﮔﺮﻓﺘﻪ ﺷﻮد.

genomic detection of coxiella burnetii in cow's milk samples of lorestan province based on is1111 transposon gene

background and aim: the present study was conducted with the aim of molecular search for coxeillaburnetii in raw milk samples collected from cows of lorestan province.materials and methods: this is a cross-sectional and descriptive studyin which 200 milk sampleswere randomly collected from the herds of traditional cattle farms belonging to two cities (khorramabad and poldakhtar) of lorestan province. collecting milk samples and recording the age of animals was done in the winter of 1400. the process of dna extraction from all milk samples was also done. then, nested-pcr reaction was used to detect c. burnetii based on the is1111 transposon gene. also, spss software and chi-square method were used for statistical analysis.results:. the results obtained from amplification of the is1111 transposon geneshowed that 5% (95% ci: 0.7-55%) of the milk samples were positive for c. burnetii microbial load. it was found that there is a significant relationship between the age of the cow and the excretion of c. burnetii through the cow milk in lorestan province (p<0.05).conclusion:. based on the findings of this study, it can be concluded that cow's milk can be considered as one of the important sources in the transmission of c. burnetii bacteria to the next hosts. therefore, because of the long shelf life of c. burnetii due to the presence of the pseudospore form, the risk of transmission of c. burnetii through raw milk and unpasteurized dairy products cannot be ignored. therefore, cow's milk should be considered as an important factor in the epidemiology of q fever and public health in the central region of lorestan province.