جستجوی مولکولی ژن‌های ( tetm, teto, tetl ) استرپتوکوکوس آگالاکتیه جدا شده از شیر بز و گوسفند دامداری‌های اطراف تهران

زﻣﯿﻨﻪ و ﻫﺪف: ﺑﻪ اﻟﺘﻬﺎب ﺑﺎﻓﺖ ﭘﺎراﻧﺸﯿﻢ ﻏﺪد ﭘﺴﺘﺎﻧﯽ، ورم ﭘﺴﺘﺎن ﮔﻔﺘﻪ ﻣﯽﺷﻮد. اﺳﺘﺮﭘﺘﻮﮐﻮﮐﻮس آﮔﺎﻻﮐﺘﯿﻪ از ﻣﻬﻢﺗﺮﯾﻦ ﻋﻮاﻣﻞ ﻣﯿﮑﺮوﺑﯽ در اﯾﺠﺎد ورم ﭘﺴﺘﺎن اﺳﺖ. ورم ﭘﺴﺘﺎن در اﻏﻠﺐ ﻣﻮارد ﻣﻮﺟﺐ، ﺣﺬف زود ﻫﻨﮕﺎم و ﮐﺎﻫﺶ ﺗﻮﻟﯿﺪ ﻣﯽﺷﻮد. ﭘﮋوﻫﺶ ﺣﺎﺿﺮ ﺑﺮای ﺑﺮرﺳﯽ ژن ﻫﺎی ﻣﻘﺎوﻣﺖ ﺑﻪ اﮐﺴﯽﺗﺘﺮاﺳﺎﯾﮑﻠﯿﻦ در اﺳﺘﺮﭘﺘﻮﮐﻮﮐﻮس آﮔﺎﻻﮐﺘﯿﮥ ﺟﺪاﺷﺪه از ﺷﯿﺮِ ورم ﭘﺴﺘﺎﻧﯽ ﮔﻮﺳﻔﻨﺪان و ﺑﺰان ﻣﻨﻄﻘ ﮥ ﺗﻬﺮان اﻧﺠﺎم ﺷﺪه اﺳﺖ.ﻣﻮاد و روش ﻫﺎ: ﺗﻌﺪاد 240ﻧﻤﻮﻧﻪ ﺷﯿﺮ ﺑﺰ و ﮔﻮﺳﻔﻨﺪ ﻣﺒﺘﻼ ﺑﻪ ورم ﭘﺴﺘﺎن از داﻣﺪاریﻫﺎی ﺻﻨﻌﺘﯽ اﻃﺮاف ﺗﻬﺮان ﺟﻤﻊآوری ﺷﺪ و ﺑﺮ روی ﻣﺤﯿﻂ ﮐﺸﺖ ﺑﻼد آﮔﺎر ﮐﺸﺖ داده ﺷﺪ. ﭘﺮﮔﻨﻪﻫﺎی رﺷﺪﯾﺎﻓﺘﻪ، ﺑﺎ ﮐﻤﮏ روشﻫﺎی ﻣﺘﺪاول ﻓﻨﻮﺗﺎﯾﭙﯽ و ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ ﺑﺮرﺳﯽ ﺷﺪﻧﺪ. در اداﻣﻪ ﺣﺴﺎﺳﯿﺖ آﻧﺘﯽﺑﯿﻮﺗﯿﮑﯽ ﺟﺪاﯾﻪ ﻫﺎ ﺑﻪ روش اﻧﺘﺸﺎر از دﯾﺴﮏ )kirby bauer( ﻣﻄﺎﻟﻌﻪ ﺷﺪ . ﺑﻪ ﻣﻨﻈﻮر ﺗﺄﯾﯿﺪ ﺗﺸﺨﯿﺺ ﺟﻨﺲ ﺑﺎﮐﺘﺮیﻫﺎی از ﭘﺮﮔﻨﻪﻫﺎی ﺧﺎﻟﺺ ﺗﺄﯾﯿﺪﺷﺪه ﺑﺎ آزﻣﺎﯾﺶﻫﺎی ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ، ﺑﺮای اﺳﺘﺮﭘﺘﻮﮐﻮﮐﻮس اﮔﺎﻻﮐﺘﯿﻪ از pcrاﺳﺘﻔﺎده ﺷﺪ و در ﻧﻬﺎﯾﺖ ﺑﺮرﺳﯽ ژﻫﺎی )teto ، tetm ،tetl( ﻣﻘﺎوﻣﺖ آﻧﺘﯽﺑﯿﻮﺗﯿﮑﯽ ﺑﺮرﺳﯽ ﺷﺪ ﯾﺎﻓﺘﻪ ﻫﺎ: ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ از ﻣﺠﻤﻮع ﺗﻌﺪاد 240ﻧﻤﻮﻧﻪ ﺷﯿﺮ ﮔﻮﺳﻔﻨﺪ و ﺑﺰ ﺑﻪ ﺗﺮﺗﯿﺐ 8ﻧﻤﻮﻧﻪ ) 6/7درﺻﺪ( و 14ﻧﻤﻮﻧﻪ ) 11/7درﺻﺪ( آﻟﻮده ﺑﻪ اﺳﺘﺮﭘﺘﻮﮐﻮﮐﻮس آﮔﺎﻻﮐﯿﺘﻪ ﺑﻮدﻧﺪ. ﻧﺘﺎﯾﺞ ﺑﺮرﺳﯽ ژن ﻫﺎی ﻣﻘﺎوﻣﺖ ﺑﻪ اﮐﺴﯽ ﺗﺘﺮاﺳﺎﯾﮑﻠﯿﻦ ﻧﺸﺎن داد، ﮐﻪ ﺗﻤﺎم ﻧﻤﻮﻧﻪ ﻫﺎی ﻣﺜﺒﺖ ﺷﯿﺮ ﮔﻮﺳﻔﻨﺪ ﺣﺎوی ژن ﻫﺎی ﻣﻘﺎوﻣﺖ ﺑﻪ اﮐﺴﯽ ﺗﺘﺮاﺳﺎﯾﮑﻠﯿﻦ ﺑﻮدهاﻧﺪ. درﻧﻬﺎﯾﺖ از ﺗﻌﺪاد 8ﻧﻤﻮﻧﻪ ﻣﺜﺒﺖ در ﺷﯿﺮ ﺑﺰ ﻓﻘﻂ ﭼﻬﺎر ﻧﻤﻮﻧﻪ ﺣﺎوی ژن )tetm( ﺑﻮدﻧﺪ .ﻧﺘﯿﺠﻪ ﮔﯿﺮی: ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﺣﻀﻮر ﻓﺮاوان ژن ﻫﺎی ﻣﻘﺎوﻣﺖ آﻧﺘﯽﺑﯿﻮﺗﯿﮑﯽ، اﺳﺘﻔﺎده ﺻﺤﯿﺢ از آﻧﺘﯽ ﺑﯿﻮﺗﯿﮏﻫﺎ و ﻏﺮﺑﺎﻟﮕﺮی ﺳﺮﯾﻊ و ﭘﯿﻮﺳﺘﮥ اﯾﻦ ﻣﯿﮑﺮوارﮔﺎﻧﯿﺴﻢ، ﺟﻬﺖ ﺟﻠﻮﮔﯿﺮی از ﻇﻬﻮر و ﮔﺴﺘﺮش اﺳﺘﺮﭘﺘﻮﮐﻮﮐﻮس آﮔﺎﻻﮐﯿﺘﻪﻫﺎی ﻣﻘﺎوم، ﺑﺎﯾﺪ ﺑﯿﺸﺘﺮ ﺑﺪان ﺗﻮﺟﻪ ﺷﻮد .

molecular detection of genes (tetm, teto, tetl) of streptococcus agalactiae isolated from milk of goats and sheep of livestock farms around tehran

background and aim: the condition of inflammation of the mammary tissue, often referred to as mastitis which canoccur due to various factors, with streptococcus agalactiae being one of the significant microbial agents responsiblefor its development. it often causes treatment costs and premature culling. the present study aimed to investigateantibiotic resistance genes for oxytetracycline in streptococcus agalactiae strains isolated from the milk of goats andsheep in the tehran region.materials and methods: a total of 240 milk samples from goats and sheep suffering from mastitis were collectedfrom industrial farms around tehran and cultured on blood agar medium. the grown colonies underwent standardphenotypic and biochemical tests. subsequently, the antibiotic sensitivity of the isolates was studied using the kirbybauer disk diffusion method. to confirm the identification of bacteria from pure cultures confirmed by biochemicaltests, pcr was employed for streptococcus agalactiae. finally, the resistance genes (teto, tetm, tetl) were examined.results: the results indicated that out of the total 240 milk samples, 8 samples (6.7%) and 14 samples (11.7%) wereinfected with streptococcus agalactiae in goats and sheep, respectively. the investigation of antibiotic resistance genesrevealed that all positive samples from sheep's milk contained resistance genes to oxytetracycline. among the 8positive samples in goat's milk, only 4 samples were found to contain the tetm gene.conclusion: considering the presence of a high number of antibiotic resistance genes, proper use of antibiotics andcontinuous and rapid screening of this microorganism should receive more attention to prevent the emergence andspread of antibiotic-resistant streptococcus agalactiae strains