effects of a luteinizing hormone-releasing hormone analogue (lhrha) on the reproductive of asian seabass (lates calcarifer)

Asian seabass (lates calcarifer), locally found in the southern waters of iran and commercially known as barramundi, is an economically valuable species due to its rapid growth, tolerance to high stocking density, resistance to adverse environmental conditions, high nutritional value, and palatability. despite these advantages, its commercial aquaculture in iran has not yet been sufficiently developed. expanding the aquaculture industry of this valuable species requires accurate knowledge of its biology and biotechnical aspects of spawning and seed production. in the present study, different doses of luteinizing hormone-releasing hormone (lhrh) analogue were tested to optimize the spawning response in male and female broodstock. the results indicated that in females with oocytes of 400 µm in diameter, injection of 50 µg/kg body weight successfully induced ovulation, while in males; a dose of 25 µg/kg body weight was suitable for spermation.