effect of resistance training on fto and ppar-y genes expression in muscle tissue of obese diabetic rats

Purpose: it has been well proven that type 2 diabetes is one of the most common non-communicable diseases leading to many complications and mortality every year. the aim of this study was to investigate the effect of resistance training on fto and ppar-y gene expression in muscle tissue of obese diabetic rats. study design: this experimental study was performed on 12 male wistar rats (10 weeks old and 220±20 g bodyweight). methods: animals received 6 weeks high-fat diet and then in order to induce type 2 diabetes an intraperitoneal injection of a single dose of 30 mg/kg freshly prepared streptozotocin (stz) (sigma, usa) solved in citrate buffer (ph 4.5) was performed. diabetic rats were divided into two (resistance and control) groups randomly. the resistance program included 6 weeks, 5 sessions per week. the muscle expression of fto and ppar-γ was measured using the real-time pcr method. independent t-test and analysis of covariance (ancova) were applied to compare the means. results: the muscle mrna expression of ppar-γ and body weight significantly increased after 6 weeks of resistance training (p=0.031, p=0.037; respectively) but there was no significant change in the muscle mrna expression of fto (p=0.317). also, blood glucose significantly decreased in the training group compared to the control group (p=0.01). conclusion: according to the results of this study, it can be noted that 6-week resistance training by decreasing glucose levels and increases insulin sensitivity, and the muscle expression of ppar-γ plays a prominent role in the control and treatment of type 2 diabetes in obese patients.‎