electrochemical determination of drugs in human plasma sample using a modified nanosensor

An extremely sensitive and selective electrochemical sensor based on anatase tio2 nanosheet hierarchical spheres (tio2nshss), pt nanoparticles (ptnps), carbon paste electrode (cpe) and 2,3-dihydro-2-(3,4-dihydroxyphenyl) quinazolin-4(1h)-one (dhp) for determination of isoprenaline (ip) in the presence of phenobarbital (pb) was developed. the excellent synergistic effect of tio2nshss, ptnps and dhp showed significantly enhanced electrocatalytic activity for ip and pb. in order to characterize the developed nanosensor, the scanning electron microscope (sem), electrochemical impedance spectroscopy (eis) and voltammetry were used. electrochemical behavior of the dhp/ptnps/tio2nshss/cpe sensor studied using differential pulse voltammetry (dpv), cyclic voltammetry (cv) and eis. several effective parameters were investigated and optimized on the nanosensor response. the proposed sensor, under the optimized conditions, showed two linear dynamic ranges from 0.05- 20.0 µm and 20.0-900.0 µm with a low detection limit of 7.0 nmol.l −1 for ip determination. finally, the modified sensor was employed to determine the analytes in human blood plasma with ±0.3% error. validation of the proposed method was performed using high-performance liquid chromatography (hplc).