bioremediation of chloride and nitrate in industrial wastewater using native enterococcus faecalis ff2021 and two algal strains

Industrial plants and microalgae require clean water for proper growth and have been increasingly explored for their roles in wastewater treatment. wastewater commonly contains anions such as chloride, sulfate, and nitrate, originating from aquaculture, hatcheries, and industrial processes. these anions can contaminate surface and groundwater, posing serious threats to aquatic life and environmental health. this study investigated the bioremediation of chloride and nitrate from aqueous environments using enterococcus faecalis ff2021, a native bacterial strain isolated from industrial wastewater, in combination with two algal species under laboratory conditions. identification of enterococcus faecalis ff2021 was confirmed through biochemical tests, pcr, and 16s rrna gene sequencing. experimental data on chloride and nitrate removal were analyzed using the ion chromatography (ic) method. to evaluate the ion-removal efficiency, factors such as culture medium, bacterial concentration, and microorganism type were assessed. the results showed that chlorella vulgaris consistently outperformed chlorococcum sp. in the removal of both nitrate and chloride ions. native e. faecalis ff2021 possesses strong ionic tolerance but limited remediation capacity alone, which can be significantly enhanced through synergistic interaction with chlorella vulgaris and chlorococcum sp. in a consortium system. moreover, integrating tolerant bacteria with high-performing microalgae might form synergistic consortia capable of improving overall bioremediation efficiency in saline or nutrient-rich industrial effluents.