thermodynamic evaluation of the effect of cu2+ ion on the interaction of ribavirin drug with bsa

In this study, the effect of cu2+ ion on the interaction between bovine serum albumin (bsa) and ribavirin drug (rib) was investigated by fluorescence spectroscopy. the negative sign of free energy (δg) confirmed that rib-bsa reaction is spontaneous both in the absence and presence of cu2+ ions. the positive values for δh and δs indicated that hydrophobic interactions play major roles in rib-bsa association in the absence of cu2+ ion, while in the presence of cu2+ ion, it is the electrostatic interaction that play essential role in the binding process.