effect of temperature and copper ion on guaifenesin-dna interaction: calculation of thermodynamic parameters

In this study, the effect of temperature and cu2+ ion on the interaction of guaifenesin (gua) with dna was investigated. the binding constant increases with the increasing of temperature, shows that the temperature has a positive effect on binding reaction. except for the temperature of 288 k, there was no significant difference between binding constants in the absence and in the presence of cu2+ ion. the negative values of δg confirmed that all binding processes are spontaneous. both δh and δs of the association reaction between dna and guaifenesin are positive, which confirmed hydrophobic interaction during binding process.