a single step and cost-effective flash nanoprecipitation method to prepare chitosan with high curcumin conjugated gold nanoparticles loading and its antibacterial investigation

A synergistic effect of gold nanoparticles and curcumin and a unique combination of chitosan natural amphiphilic polymer due to their widespread biological and technological applications have gained much attention. their simpler synthesis for encapsulating curcumin-conjugated gold nanoparticles in particulate carriers assembled by polymer as trapping agent via green chemistry has also become of foremost importance. the current study offers to report a new, simple, single-step, and cost-effective room temperature strategy for the fabrication of curcumin-conjugated spherical gold nanoparticles dispersed on the surface of chitosan by ph adjusting that curcumin acts as the reducing and stabilizing agent based on the flash nanoprecipitation.  the prepared samples were characterized with uv-vis, afm, and tem analysis. regarding the obtained data from the antibacterial test by disc diffusion method, the prepared chitosan with high curcumin conjugated gold nanoparticles compared to the pure chitosan and curcumin encapsulated chitosan nanoparticles with high antibacterial activity will open a unique opportunity for industrial scale-up. the inhibition zone diameter was 25 mm which is more than the inhibition zone diameter for chitosan (7 mm) and chitosan with curcumin loading (12 mm).