immobilization of laccase nanoflower in polydopamine biofilm for biocathode formation and its integration with glucose dehydrogenase bioanode in a membrane-free of glucose/o2 biofuel cell

In this research, a glucose/o2 biofuel cell was developed with glucose dehydrogenase bioande and laccase biocathode. laccase nanoflowers were immobilized onto gold nanoparticles, which were electrodeposited on a gold electrode and polydopamine biofilm was employed to keep the immobilized laccase nanoflowers in place. the biocathode illustrated a high electrocatalytic activity toward oxygen reduction reaction with a direct electron transfer at a positive potential of +0.61 v (vs. ag/ agcl). for the preparation of a bioanode, glassy carbon electrode was modified in a layer by layer structure of graphene oxide nano-sheets, glucose dehydrogenase, and nicotine amide adenine dinucleotide by electrostatic affinities and the capping layer consist of glutaraldehyde, bovine serum albumin and glucose dehydrogenase. the proposed system depicted a high bioelectrocatalytic activity toward glucose oxidation and the oxidation commenced at -0.30 v (vs. ag/ agcl).