the effect of the presence of chondroitin sulfate on porosity and mechanical properties of enzymatically cross linked silk fibroin/ chondroitin sulfate hydrogel

Conjugation of tyrosine groups in the presence of horseradish peroxidase (hrp) and hydrogen peroxide (h2o2) as a catalyst have formed in situ-forming, injectable, enzymatically crosslinked hydrogels. in the current study, we have synthesized the injectable hybrid hydrogel comprising silk fibroin (sf) and chondroitin sulfate (cs) through the di-tyrosine formation bond of sf chains. altering the chondroitin sulfate concentrations (i.e. 0, 0.25, 0.5, 1, 2 wt.%) which are interpenetrated between silk fibroin β-sheets and chains potentially adjusts the physical, chemical, and mechanical features of these hybrid hydrogels. the results showed the addition of 0, 0.25, 0.5, 1, and 2 wt. % cs to sf could increase the compression strength of the sf/cs hybrid hydrogels along with the increase in porosity.