effect of ajowan (trachyspermum ammi) on hydatid cyst protoscoleces: an experimental study

Hydatid cyst disease poses a serious health issue worldwide. protoscoleces, essential stages in the parasite’s life cycle, are critical targets for therapeutic research (1). this study assessed the impact of ajowan (trachyspermum ammi), a plant-based compound, on the mortality of hydatid cyst protoscoleces (2, 3). protoscoleces were extracted from hydatid cysts and kept in phosphate-buffered saline with a ph of approximately 7.40. they were treated with ajowan at concentrations of 10, 1, 0.10, and 0.01 µg/ml for durations of 1, 30, 60, and 120 min. after that, the protoscoleces were stained with 1% eosin and analyzed under a light microscope to determine mortality rates. the study results revealed a significant increase in protoscolex mortality with higher ajowan concentrations and longer exposure times. at 1 µg/ml, mortality reached 100% after 30 min. lower concentrations required more extended treatment; 80% mortality was achieved at 0.10 µg/ml after 60 min, and 50% at 0.01 µg/ml after 120 min. statistical analysis confirmed that these differences in mortality rates across various concentrations and durations were statistically significant. these results underscore the potent effect of ajowan on hydatid cyst protoscoleces, even at low concentrations and brief treatment times. the study concludes that t. ammi shows considerable promise as a therapeutic agent for managing hydatid cyst disease. this research lays the groundwork for further studies and development of new treatments for this parasitic infection.