investigation of the effects of rutin against diabetic nephropathy in rats

Diabetes mellitus is a disease characterized by excessively high blood sugar levels causing complications in many systems. diabetes mellitus can lead to disorders in carbohydrate, fat and protein metabolism as well as serious health problems such as neuropathy, retinopathy, nephropathy, peripheral vascular disease, coronary artery disease and atherosclerosis. diabetic hepatopathies are a common chronic complication affecting approximately 30% of patients with diabetes. rutin (quercetin rutinoside) is a glycoside of the flavonoid quercetin. rutin prevents platelet aggregation has anti-inflammatory effects and reduces circulating fat and cholesterol. in this study, we aimed to determine the effects of rutin against nephropathy due to dibetes mellitus. for this purpose, 40 female sprague-dawley rats were randomly divided into 4 groups as control, diabetes, diabetes+metformin and diabetes+rutin50 groups. at the end of the study, rats were sacrificed and kidney tissue samples were taken. in the diabetes group, degeneration, necrosis, glomerular atrophy and hyperemia were observed in the renal tubule epithelium due to hyperglycemia while these pathological conditions were significantly reduced in the routine treatment group. in addition, immunohistochemical and immunofluorescence analyses revealed that the expression levels of tim and 8-ohdg which were at very high levels due to diabetes,were decreased significantly. as a result of the examinations, it was determined that rutin treatment reduced apoptosis and dna damage by reducing oxidative damage in kidney tissues and increased tissue resistance by strengthening immunity. this suggested that rutin had alternative or complementary effects in the treatment of diabetes. we believe that it will shed light on future studies.