benefits of combining piperine with prednisolone in an experimental model of rheumatoid arthritis

Anti-inflammatory and immunomodulatory properties of piperine have been documented. this study evaluated the beneficial effects of piperine on clinical symptoms and immune responses in wistar rats with rheumatoid arthritis (ra) induced by freund s complete adjuvant (cfa). the ra rats were randomly divided into three groups (n = 10): ra rats treated with pbs (100 mg/kg orally), ra rats treated with piperine (100 mg/kg orally), and ra rats treated with prednisolone (10 mg/kg orally). treatment commenced on day five post-induction when all rats had a clinical score of ≥1. disease symptoms were monitored every other day until day 23 post-induction. treatment with piperine resulted in a significant reduction in disease severity and improved weight gain in ra rats. biochemical indices of crp, myeloperoxidase and no were significantly decreased in the treatment group compared to the non-treatment group. the group treated with piperine exhibited a significant decrease in the expression of t-bet, gata-3, and rorɣt genes compared to ra rats without treatment. however, there was no statistically significant difference in foxp3 gene expression in the piperine-treated group compared to the untreated group. overall, piperine might represent a valuable approach to managing ra.