the assessment of in vitro timosil drug release through dialysis bag

Understanding drug release in poultry s digestive system is crucial for effective disease treatment and feed efficiency optimization. improper release can lead to side effects, like gastrointestinal irritation or toxicity (1). understanding the mechanisms can help develop formulations minimizing adverse effects, ensuring the safety of final poultry products. the ph levels in the digestive tract significantly impact drug delivery and absorption (2). designing appropriate drug delivery systems can improve the efficacy and bioavailability of medications in poultry production. this experiment evaluated the release of commercial drug in different parts of the poultry s digestive tract. the kinetics of drug release in phosphate-buffered saline at the specific ph (crop and proventriculus: 2.50-4.50; gizzard: 2.50-3.50; small intestine: 6.00-7.50; cecum: 6.00-7.00) at 41˚c were investigated (3). in time intervals of 30 to 480 min, 2 ml of the buffer was removed each time and 2 ml of the new buffer was replaced. the absorption of the samples at a wavelength of 250 nm was checked by a spectrophotometer. the results showed that the release rate of drug increased over time. during the investigation of the release in the phosphate buffer, it was found that about 72.00% of the drug was released in 8 hr (ph: 5.50). as a result, the release of timosil drug in acidic ph had the lowest rate, indicating that the release of drug was more in the terminal parts of the digestive tract, where the absorption is higher.