effect of rutin/cyclodextrin ınclusion complex on short-term storage of ram semen

Rutin is one of the bioactive flavonoid compounds found in many fruits and vegetables. numerous pharmacological characteristics of rutin, including anti-bacterial, anti-oxidant, anti-inflammatory and immunomodulatory effects have been reported previously (1). however, rutin s low water solubility, limits its systemic bioavailability (2). cyclodextrins (cds) are natural cyclic oligosaccharides obtained from the enzymatic breakdown of starch (3). the cds act as complexing compounds increasing the permeability and adsorption of a substance across the membrane barrier, improving chemical stability. in the present study, it was aimed to investigate the effect of the rutin/cd inclusion complex on spermatological parameters during the short-term storage of ram semen. eight tuj breed rams being free of systemic and genital organ diseases with the age of 1.50-2 years were used as animal material. ejaculates were taken from each ram by artificial vagina during non-breeding season. the semen was divided into 4 equal parts as control, 0.50 mm rutin/cd, 1 mm rutin/cd and 1.50 mm rutin/cd groups. after the addition of rutin/cd to the groups, the temperature of all groups was gradually reduced from 37 to 5˚c. analyses of semen at 0, 24, 48, 72 hr and oxidant-anti-oxidant capacity (tos/tas) at 72 hr were carried out. when the results were analyzed, an increase in tas, a decrease in tos and an increase in sperm quality were observed in 0.50 mm rutin/cd group. in conclusion, rutin/cd administered to rams sperm at a dose of 0.50 mm improved semen quality during short-term storage of semen.