histopathological evaluation of the effect of oral administration of sericin on the bone defect healing in rat

This study investigated the effects of silk sericin on bone healing in rats considering its potential applications in biomedicine. fifty male wistar rats were randomized into five groups. after anesthesia, a cavity was created in the right femur using a dental drill (1). the sham group underwent surgery without a defect, while the control group had an untreated defect. experimental groups received daily oral doses of sericin at 100, 150, and 200 mg/kg (2). histopathological evaluations showed that the control group defect was filled with connective tissue, with newly formed trabecular bone observed deeper within (3). in the 100 mg sericin group, granulation tissue was more organized, filling most of the defect and showing early cartilage formation. the 150 mg group exhibited organized granulation tissue and significant dense bone formation beneath the fibrous tissue, effectively blocking the defect and severing the connection to the bone marrow. the 200 mg group also showed organized granulation tissue, advanced bone remodeling and the formation of haversian systems at the repair site. overall, the results indicated that oral sericin administration significantly enhanced tissue healing leading to optimal recovery from bone injuries in rats. this research highlighted sericin effects as a therapeutic agent in bone healing applications.