effects of quercetin against deoxynivalenol induced adverse effects on intestinal epithelial cells

The hct116, a human colon cancer cell line often used in colon cancer research, was employed to investigate the protective effects of quercetin, an anti-oxidant present in various foods, against deoxynivalenol s harmful effects on these cancer cells. the study exposed the cells to increasing quercetin concentrations before simultaneous exposure to quercetin and deoxynivalenol. cell viability was assessed using the mtt test, and oxidative and nitrosative stress levels were measured. results showed a significant decrease in cell survival when exposed to deoxynivalenol compared to the control, while cells treated with quercetin exhibited notably higher survival rates. groups treated with 50 and 100 μm quercetin demonstrated the ability to decrease malondialdehyde levels and enhance total anti-oxidant capacity compared to the deoxynivalenol control group. moreover, various concentrations of quercetin were found to lower total lipid peroxidation levels in comparison with deoxynivalenol control group. overall, quercetin exhibits the potential to mitigate the effects of deoxynivalenol in hct-116 cell lines and may be considered for further investigation as a supplementary agent in the context of the aforementioned cancers.