a novel electrospun-based sorbent for electrically assisted solid phase micro extraction of six non-steroid anti-inflammatory drugs and simultaneous determination of them in human plasma samples with hplc-uv

Development of novel electrospun nanofibers as a sorbent for electrically assisted solid phase micro extraction (ea-spme) of nsaids is essential for sample pre-treatment. herein, firstly, a novel electrospun nanofibers (pva/casein/pani/tio2 nano particles) was fabricated for ea-spme on pewter rod; and then, trace amounts of six nsaids (acetaminophen, caffeine, naproxen, celecoxib, ibuprofen and mefenamic acid) were extracted. finally, these drugs were determined from different human plasma samples with hplc-uv. from the characterization of pva/casein/pani/tio2 nano particles electrospun nanofibers with field emission-scanning electron microscopy (fe-sem), energy-dispersive x-ray spectroscopy with elemental mapping analysis (eds-mapping), fourier transform-infrared (ft-ir) and x-ray diffraction (xrd), the synthesis of composite nanofibers was approved. in addition, casein that was existed in composite nanofibers, has many functional groups and it is a polar molecule that we extracted it from expired milk with acetic acid but it could not preparate electrospun nanofibers alone and pva must be mixed with it [1]. due to the nsaids are polar molecules; so, there are interactions between electrospun nanofibers and analytes. also, the electrochemical synthesis of pani on platinum disk electrode by chronoamperometry and use of it in composite nanofibers, leads to that the used pewter rod in ea-spme was more conductive for using of it as a working electrode at electrochemically desorption with chronoamperometry technique. but in this work the adsorption was done chemically [2]. also, tio2 nanoparticles was used for increasing the porosity of electrospun nanofiber [3] but the selectivity of this technique did not change. the optimum percent of additive compound to casein in order to electrospinning, was reached from design-expert 12.0 software. also, the effective factors on adsorption and desorption process were optimized by one at time (oat) method. according to optimum conditions, the wide linear range was obtained with coefficient of determination (r2) ≥ 0.99, low limits of detection based on s/n=3 and large enrichment factors. finally, the efficiency of the ea-spme-hplc-uv method was evaluated for the determination of nsaids in human plasma samples with good recoveries.