polydopamine imprinted polymer-based tunable electrochemical synthesis of cu-btc mof film as a hybrid dual recognition element for ultra-trace sensing of pregabalin (lyrica)

Abstract: the present study propounds an innovative diagnostic hybrid dual recognition strategy for detecting trace amounts of pregabalin (pgb) using the combination of two powerful metal-organic framework (mof) structures and the molecularly imprinted polymer (mip) method [1]. to improve the electrode analytical performance, copper-benzene-1,3,5-tricarboxylic acid frameworks (cu-btc mofs) were synthesized directly on the electrode through three stages by electrodeposition of cu nanoparticles on gce and direct chemical conversion of them to copper hydroxide nanotubes (cu(oh)2 nts) intermediate and then swiftly chemically convert nts to cu-btc mof layer[2]. then mip film was synthesized via the in-situ dopamine (da) electropolymerization [3]. surface morphology was characterized by field emission scanning electron microscopy (fesem) images at all stages. to explore the effect of independent variables on the mip construction, the box-behnken design was considered to determine response surface methodology[4]. in this sense, the predicted optimum combination variables were da=3 mm, ph = 7.4, cycle number = 16, koh 4 mm, koh: et-oh ratio of 5:1 and 18 min elution time. incubation time was obtained at 12 min. electrochemical impedance spectroscopy (eis) was used to determine the pgb analyte in experimental stages. in addition, in optimal research conditions, analytical parameters such as linear range, accuracy and precision, were explored. the linear dynamic range and lod were 0.05- 800 pm and 2.9 fm respectively. the imprinting calculated factor was 24.60, showed good interaction between the pgb and da monomer. inter-day and intra-day reproducibility measurements showed 2.08% and 1.58% and, 98% of the initial current response was maintained. coefficients of selectivity against the pgb, for tapentadol, gabapentin, levetiracetam, l-carnitine, and l-histidine were 4.20, 0.76, 0.48, 1.50, 0.64 and 0.64. cv scanning at various scan rates verified that adsorption kinetics follows a diffusion control mechanism. finally, the gce/cu-btc/epda-mip sensor's efficiency in urine and human serum samples was evaluated, and satisfactory results were achieved.