determination of “glycolipid” as biosurfactant in hair baby shampoo with lectin base affinity chromatography

Abstract: biosurfactants are surface-active molcules produced by microorganisms that have been highlighted as an environmentally-friendly alternative to their synthetic counterpart, chemical surfactants in hair and skin products. the emulsifier function is probably the most important property of biosurfactants in the formulation of cosmetics, because emulsions have considerable advantages over other types of materials. recently more solvent systems have been described for the separation of glycolipids, quantitative analysis with chromatography methods has been recognized to be difficult even with post-column detection by ultraviolet (uv), refractive index (ri), and fluorescence detectors. in this work, we reviewing several extraction and analysis methods for biosurfactants in cosmetic products [1-4], finally we examined the amounts of biosurfactants “glycolipid “used in baby shampoo. due to the appropriate interaction of this type of biosurfactant with lectin extracted in the plants, we succeeded in using the optimized affinity chromatography method in the capillary column to separate and determine the exact amount of this biosorfactant with using tlc. results showed that an approximate error of 0.2% with achieve a linear concentration range of 2-13% in the formulation of various baby shampoo products. this method used for determining the amount of several baby shampoos containing glycolipid biosurfactant were analyzed and compared with previous methods, that demonstrate no significant difference was observed in the results. the present method provides easy and usability for other cosmetic products with an acceptable linear range.