pvc/mg-al ldh electrospun nanofibers as a new sorbent for simultaneous micro-spe and determination of da and ua in human blood plasma samples followed by sensitive voltametric biosensor (gce/ppy/dea mip) detection

Abstract: dopamine (da) and uric acid (ua) always coexisted in the extracellular fluids of the central nervous system and serum in mammals, which play critical roles in human metabolism [1-3]. new electrospinning nanofibers with polyvinyl chloride (pvc)/a layer of double hydroxide (mg-al-ldh) were prepared. since measuring trace concentration of da and ua in complex matrix is essential, we used electrospinning method for preparing nanofibers and then used nanofibers as a new sorbent in micro extraction method for simultaneous extraction of da and ua prior detection with glassy carbon electrode (gce)/ poly pyrrole (ppy) /da, ua, and diethyl amine (dea) as molecularly imprinted polymer (mip). effective factors on adsorption process; including ph of adsorption solution, adsorption time, percentage of salt added to the adsorption solution, stirring rate, and the size of sorbent, were optimized by the one-at-time (oat) method. effective factors on constructed biosensors such as time and potential and effective factors on desorption process; including desorption time, eluent type, and the volume of eluent, were optimized by design-expert-12.0 software. the nanofibers and biosensor were characterized by fe-sem, xrd, and ft-ir. finally, under optimal conditions, the electrochemical biosensor was put into human blood plasma samples, and the standard addition method was applied with the spike of da and ua to them. the wide linear range and lower detection limit were obtained.