distinguishing of autism disorder by the aptasensor based on screen printed carbon electrode modified with gold nanorods

Autism spectrum disorder (asd) is a complex neurodevelopmental disorder among children that is estimated to a ration of 1 in 160 in children all over the world by the world health organization (who) reports [1]. the higher levels of bovine β-casomorphin 7 (bcm-7) biomarker in autistic children's urine than in healthy children is correlated with the severity of the autistic symptoms. despite some reported spectroscopic and chromatographic-based methods in bcm-7 detection, some practical disadvantages such as complexity, need for skilled expertise and even the inability to trace bcm-7 in the biofluids limits their applications [2,3]. to overcome these issues, developing an ultrasensitive and selective aptasensor for the accurately sensing of bcm-7 in human biofluids has great significance in asd distinction. an electrochemical aptasensor has been developed based on a screen-printed carbon electrode (spce) modified with gold nanorod (aunr). aunr not only suggested a large specific surface area on the spce surface but also captured more apt sequences by forming a chemisorption interaction between the amine-au groups. by bcm-7 introducing onto the sensing interface, the apt configuration was changed to form a bcm-7/apt complex. the induced spatial barrier on the surface created a linear relationship with bcm-7 concentration increasing over a dynamic range of 1 fm to 25 nm with a limit of detection (lod) of 334 am. the well-evaluated applicability of the aptasensor in the presence of some interfering species and also in some human urine and plasma samples as actual samples can be promised the autism distinguish as early as infancy under clinical conditions [4]. the proposed strategy may be broadly applicable for fabricating other miniaturized biodevices because of utilizing cost-effective spces to economically reduce the cost and replace the apt sequence as the bioreceptor for different targets.