syringe to syringe-dispersive liquid–phase micro extraction combined with uv-vis spectrophotometry for pre concentration and determination of acetaminophen & codeine phosphate with the aid of experimental design

This study was aimed at developing a new, technically simple method for pre concentration and determination of acetaminophen and codeine phosphate combination by syringe to syringe-dispersive liquid–phase micro extraction technique combined with uv-vis spectrophotometry method and compared with common brand names include tylenol and panadol. the study assessed factors affecting on the extraction technique, extraction solvent [1], extraction solvent volume [2], ph of the solvent, effect of sample ph and centrifugation time, ionic strength on the extraction of target analytes, extraction time, effect of syringe to syringe numbers, temperature, sample to solvent ratio, sample characteristics and are the defining factors that effect extraction efficiency, effect of ionic strength on the extraction of target analytes, effect of syringe to syringe numbers, also effective factors were screened using a response surface methology (rsm) and optimized using a i-optimal. under the optimized experimental conditions, the calibration curve was a dynamic linear in the range for the assay of acetaminophen (5.0 – 25.0 mg l-1) and codeine phosphate (1.25 – 10.0 mg l-1) and the limit of detection (lod) for the acetaminophen (0.15 mg l-1) and for the codeine phosphate (0.26 mg l-1) and limit of quantification (loq) for the acetaminophen (0.50 mg l-1) and for the codeine phosphate (0.87 mg l-1) with r2 = 0.9991 for acetaminophen and r2 = 0.9995 for codeine phosphate respectively. the absorption maxima of drugs in mixture were found to be at 243 nm for the both of acetaminophen and codeine phosphate respectively, in 1-dodecanol as extraction solvent. the proposed method was applied to the determination of codeine phosphate and acetaminophen in raw material and solid dosage forms (powder and granules tablets and capsules) and urine samples.