the combination of dispersive liquid-liquid microextraction method with thin film evaporation for extraction of rivastigmine drug in biological samples before analyzing by secondary electrospray ionization-ion mobility spectrometry

The combination of dispersive liquid-liquid microextraction method with diffusive thin film as a surface for evaporation of solvent was used to extract and preconcentration of rivastigmine drug. secondary electrospray ionization-ion mobility spectrometer apparatus was applied to identify of rivastigmine molecules. the thin film used in this method was prepared using electrospinning method. to measure the trace amount of rivastigmine and achieve the highest extraction efficiency, some effective parameters such as type of extraction and disperser solvents, volume of extraction and disperser solvents, centrifugation time, solvent evaporation time from the film surface and sample ph were studied. type of extraction solvent; chloroform, type of disperser solvent; methanol, volume of extraction solvent; 90 microliters, disperser solvent volume; 1.5 ml, evaporation time from the film surface; 1 minute, centrifugation time; 5 minutes and the ph of the sample solution; 10 was obtained as the optimized conditions. under the optimized conditions, the limit of detection (lod) and linear dynamic range (ldr) were obtained 3 µg l-1, 10-1000 µg l-1, respectively. the intra- and inter-day relative standard deviations were calculated to be 4% and 6%, respectively. the capability of the proposed method for analyzing of rivastigmine drug in the urine, saliva, and plasma samples were investigated and the relative recovery values were calculated 101-113%.