salting-out assisted micro liquid-liquid extraction (sa-µ-lle) of sertraline antidepressant from different real samples and determination by uv-vis spectrophotometry

Sertraline is an antidepressant with selective inhibition of serotonin reuptake, which decreases serum level of inflammatory factors in patients with depression [1]. salting-out assisted micro liquid-liquid extraction (sa-µ-lle) is a simple extraction method which used polar and simple solvents (such as acetonitrile) for extraction of different analytes [2]. in this research, sa-µ-lle combined with uv–vis spectrophotometry was applied for extraction and determination of sertraline. sertraline is a secondary amine and it forms dithiocarbamate complex in the present of carbon disulfide (cs2) and nickel(ii) ion. the formed complex has maximum wavelength at 389 nm. the effective factors such as ph, type and volume of extraction solvent, effect of type and amount of salt, and derivatization parameters were investigated and optimized. the best condition was achieved by tetrahydrofuran as the extraction solvent, nacl as the salt, at ph equal to 11. under optimal conditions, the linear concentration was from 1 to 20 µg/ml, the intra- and enter-day precisions were 6.3 and 8.5% respectively. finally, the method was applied for determination of sertraline in the pharmaceutical formulation and urine samples. the relative recoveries were in the range of 86 to 112%.