an “on-off” sensor based on silver nanoprisms for the determination of phenytoin sodium in exhaled breath condensate

Abstract: in the current study, a fluorescence-based detection system that uses silver nanoprisms is proposed for determination of phenytoin sodium. this probe is based on turning off and turning on the fluorescence emission related to luminol, which is performed by nanoprism and phenytoin sodium, respectively. energy transfer from luminol to silver nanoprism reduced its fluorescence emission intensity. phenytoin sodium adding cause the silver nanoprism aggregation and inhibits the fluorescence quenching of luminol and fluorescence intensity is increased in proportional with phenytoin sodium concentration. the experimental condition are optimized using one-a-time method and full validated in exhaled breath condensate as a chosen biological sample. the validated method is capable of technically detecting the phenytoin sodium in the range of 0.01 to 2.0 mg.ml-1 with limit of detection of 0.005 mg.ml-1 [1].