bubble-in-drop microextraction of medicinal plant followed by gas chromatography-mass spectrometric analysis

Bubble-in-drop microextraction (bid) is a variation of single-drop microextraction in which an air bubble is deliberately introduced to the solvent to enhance extraction efficiency. here, bid coupled with gc-ms was developed for the extraction, enrichment and determination of essential oils of medicinal plants [1]. this article investigates the effect of microwaves on the amount of volatile compounds echinophora platyloba dc with two methods, headspace single-drop microextraction (hs-sdme) and bubble-in-drop headspace microextraction (bid-sdme), for the analysis of essential oil [2]. solvent selection, solvent volume, microwave power, irradiation time and sample mass were optimized by simplex method. thirty two components were extracted and identified by use of this method, e-β ocimene (37.54%), r-d-decalactone (14.06%), α-pinene (8.35%) and nonane (6.85%) were the major constituents. it was observed that relative standard deviation (rsd) values for the main compounds were less than 9.5%.