evaluation of effective healing process of surgical wounds by hydroxypropyl methyl cellulose nanogel containing zataria multiflora essential oil coated with polycaprolactone/chitosan nanofibers

Aim and background: skin plays an important role in human health and its defects can cause great problems. thyme plant is one of the very useful herbal medicines that have various properties such as anti-inflammatory and antibacterial. therefore, the properties of this plant have made this drug a suitable candidate for wound healing. in this study, a shirazi thyme volatile oil nano gel (nezm) with a nanofibrous scaffold was used to investigate wound healing. methods: thyme nanoemulsion was prepared using the ionic gelation technique and then it was synthesized as nano gel by hydroxypropyl methyl cellulose (hpmc). the size of the nano gel was measured by the dls method. pcl-cs nanofiber scaffold was also synthesized by electrospinning method and its morphology was measured by sem. to investigate the effect of this scaffold on wound healing, several 24 rats were in 4 groups 1. control, 2.nezm nano gel, 3.pcl-cs nanofiber+nezm nano gel, 4. pcl-cs nanofiber was examined in terms of histology (inflammation, edema, granulation tissue, and fibrotic tissue) on days 7, 14, and 21.  results and discussion: the results showed that the nano gel had a size of 216 ± 8 nm. nanofibers synthesized by the electrospinning method also show smoothness without beads. the histological results also show that the pcl-cs+nezm group was able to significantly reduce the parameters of edema, and inflammation and increase the parameters of fibrosis and collagen deposition compared to other groups on days 3, 7, 21. conclusion: the results of this study show that the nezm nanogel+ pcl-cs nanofiber group can effectively improve wound healing