the most effective electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

Aim and background: the integration of biomaterial conductive scaffolds and electrical stimulation (es) has been found to significantly enhance the differentiation of stem cells into electro-responsive cells such as neural cells. this research endeavors to produce pcl/ppy nanofiber scaffolds using the electrospinning technique, and to examine how the duration of es influences the neural differentiation of conjunctiva mesenchymal stem cells (cjmscs). methods: the scaffold s topography was analyzed through sem and tem microscopy and ftir. cjmscs were cultivated on the scaffold before being exposed to electrical current at 115 v m−1 at 100 hz for 1 minute per day for a week. the impact of the nanofiber scaffold and electrical current on cell viability and neural marker gene (nestin) expression was examined via mtt assay and qpcr analysis.results and discussion: the biocompatibility of the pcl-ppy nanofiber scaffold was found to be satisfactory in our study. furthermore, the results of q-pcr analysis showed that electrical stimulation for a duration of one minute per day over a period of seven days can promote neural differentiation of cjmscs, as evidenced by a significant increase (approximately 213-fold) in the expression level of nestin gene.conclusion: this study highlights the potential applications of combining electrical current with an electrically conductive nanofibrous scaffold in the field of neural tissue engineering.