molecular docking and in-vitro tyrosinase inhibitory potential of gallic acid and its nanoniosome

Aim and background: melanoma is a type of skin cancer with high mortality. this study aimed to investigate the inhibitory effect of nanoniosome containing gallic acid on melanogenesis and gene expression of the melanogenesis pathway of melanoma cell lines b16f10. methods: at first, molecular docking of gallic acid against tyrosinase was evaluated by autodock 4.2. melanoma cell lines b16f10 were purchased from the pasteur institute cell bank and cultured in a culture medium containing 1% antibiotic and 10% fetal bovine serum at 37₀ c. the cell viability of b16f10 was assessed after treatment with different concentrations of gallic acid and its nanoniosome (10, 20, 40, 60, 80, and 100 μm). furthermore, their inhibitory effect on tyrosinase activity and tyrosinase expression were investigated by spectrophotometry and real-time pcr, respectively.results and discussion: docking results showed hydrophobic and hydrogenic interactions between ligand and tyrosinase with δg=-133.26 kcal mol-1. the results showed that gallic acid and its nanoniosomes act as melanogenesis inhibitors. conclusion: our study showed that gallic acid nanoniosome might be more effective than gallic acid for treatment of melanoma and pigment disorders such as hyperpigmentation