in vitro effect of peganum harmala l. seeds extract on resistant colorectal cancercells

Oxaliplatin is an effective chemotherapeutic medication for the treatment of colorectalcancer (crc), however prolonged usage of it frequently leads to drug resistance. it iscritical to develop methods for reducing crc cell oxaliplatin resistance. peganumharmala l. (p. harmala), is widely used in traditional medicine as an anticancerremedy. in present study, oxaliplatin-resistant hct116 cells were treated with varyingdoses of aqueous extract derived from p. harmala seeds for 24 and 48 hours. themethylthiazol tetrazolium (mtt) assay was used to determine cell viability. theanalysis showed a significant relationship between the increase in the number of deadcells and the increase in the concentration of p.harmala’s aquaous extract.the cytotoxicity of p. harmala extract increased significantly from 24 h to 48 h inoxaliplatin-resistant hct116 cell line. these findings suggest that p.harmala’saquaous extract, may be useful for treatment of drug-resistant colorectal cancer.