high-resolution ultrasound imaging for non-invasive characterization of acute wound healing in radiation injury on guinea pig skin tissue

Purpose: high-resolution ultrasound imaging is a non-invasive and objective appraisal. ultrasound imaging accomplishes the target assessment and follow-up of radiation-induced skin injury.the study aimed to investigate the complete anatomical and structural alternations of acute wound healing in skin tissue radiation injury after cell therapy with high-frequency ultrasound imaging techniques.materials and methods: female guinea pigs (250 g) were divided into 3 groups: (a) controls, consisting of non-treated guinea pigs; (b) radiation-treated; (c) radiation-treated receiving adipose-derived mesenchymal stem cells. acute radiation-induced skin injury was induced by a single fraction of x-ray irradiation of 60gy to a 3.0×3.0-cm area with a 1.3-cm bolus on 100-cm ssd in the abdominal skin tissue. ultrasonic imaging of the depth and quality of healing in the skin tissue was performed by processing ultrasound images at 40-mhz and 75-mhz frequencies.results: skin thickness indicated a significant difference between the treatment and control groups on day 10 after 60 gy irradiation (p<0.05). the highest skin thickness was observed in the irradiated group, and the lowest skin thickness was found in the stem cell treatment group.conclusion: evaluation of skin thickness, wound depth, and scar formation is important for the proper assessment and management of wound healing in stem cell therapy of radiation-induced skin damage. high-resolution ultrasound at 40- and 75-mhz frequencies is a major non-invasive method providing unprecedented insight into determining the characterization of the skin, particularly in the context of wound healing.