can heterogenic patterns of jak2, mpl, and calr genes predict specific clinical characteristics of myeloproliferative disorders?

Myeloproliferative neoplasm (mpn) is a neoplasm with three categories; essential thrombocythemia (et), polycythemia vera (pv), and primary myelofibrosis (pmf) and it usually is diagnosed through mutation analysis in several essential genes; jak2, mpl, calr. the mutations of mentioned genes in 50 patients with mpn and 50 healthy volunteers were determined via allele-specific pcr and sequencing. based on the results, mpn and its subtypes have significant relation with mutations (p<0.05). jak2 (exon 14) mutation was related to mpn and its subtypes except for et and calr (exon 9) type 1 was merely related to et, but calr (exon 9) type 2 mutation was more prevalent in mpn and pv (p<0.05). none of the mutations co-occurred simultaneously. there was no evidence of mutation in jak2 (exon 12) and mpl (exon 9 and 10) in our study, so they are unsuitable diagnostic candidates. so, mutations in jak2 (exon 14), and calr (exon 9) type 1 and 2 are essential in mpn diagnosis in iranians.