adjustment of a fibrosis marker, pro-inflammatory cytokines, and ige in asthmatic animals

Background: a lot of patients are suffering from asthma. for decreasing the asthma symptoms, we studied the effects of conditioned medium (cm) of human amniotic membrane mesenchymal stem cells (ham-mscs) as a source of anti-inflammatory cytokines on splenocyte and lung tissue of asthmatic balb/c mice. methods: forty mice were categorized into four groups; ovalbumin (ova)-induced asthma, cm-treated asthma, dmem (dulbecco’s modified eagle medium)-treated asthma, and saline control. each group received related treatment. the lung alpha-smooth muscle actin (α-sma)  and splenocyte inflammatory cytokines and ige were examined through western blot analysis. results: western blot showed α-sma overexpression in the ova and dmem groups compared with the saline group. cm therapy could significantly reverse it compared with ova and ova+dmem categories by elevating il-10 and ifn-γ and reducing il-4, ige, and tgf-β . conclusion: cm treatment could improve asthma symptoms by adjusting α-sma in lung tissue and pro-inflammatory cytokines and ige in splenocytes.