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evaluation of v617f jak2 gene mutation by high-resolution melting method in patient with erythrocytosis
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نویسنده
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mohammed fawziah m. ,esfahani vahid reza ,pakzad hossein
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منبع
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personalized medicine journal - 2020 - دوره : 5 - شماره : 17 - صفحه:9 -11
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چکیده
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Although several techniques have been developed for the detection of jak2 v617f mutation, these techniques have their disadvantages. high-resolution melting (hrm) analysis is a new, post-pcr analysis. simple and fast, this method is based on pcr melting curve techniques. this study examined the jak2 v617f mutation by the high-resolution melting method in 20 patients with erythrocytosis, and the results were compared with those obtained from the direct sequencing method. the results showed 100% sensitivity and 100% positive predictive value for this methodology in the patient sample set tested.
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کلیدواژه
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myeloproliferative ,jak2 ,point mutation ,high-resolution melting
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آدرس
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kuwait medical laboratory sciences, faculty of allied health sciences, kuwait, personalized medicine research center of amitisgen, iran, islamic azad university, tehran-east branch, department of biology, iran
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Authors
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