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comparison of different methods of dna extraction from paraffin-embedded tissues
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نویسنده
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megdadi saeed ,shojaei najme ,ibrahim ramadhan ,bakhshandeh bavarsad sareh
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منبع
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personalized medicine journal - 2020 - دوره : 5 - شماره : 17 - صفحه:5 -8
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چکیده
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The most common human archival specimens are formalin-fixed and paraffin-embedded tissues. pcr-based techniques have been coupled with new developments in the extraction of dna from ffpe. herein, we report the results of a comparison of different methods of dna extraction from ffpe specimens, including phenol-chloroform, salting-out, and silica-based commercial kits. results showed no significant differences between the amounts of dna obtained from each of the extraction methods studied; however, the salting-out dna extraction method described is much easier and less toxic than the phenol–chloroform method.
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کلیدواژه
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paraffin-embedded tissues ,dna extraction ,salting-out ,phenol-chloroform
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آدرس
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nourdanesh institute of higher education, department of biology, iran, personalized medicine research center of amitisgen, iran, salahaddin university, college of agriculture, fish recourses and aquatic animals department, iraq, university of shahrekord, faculty of management, department of public management, iran
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Authors
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