مطالعه کمی و کیفی آنتی بادی igy طیور بدست آمده از تخم برخی پرندگان اهلی و زینتی

ﭘﺮﻧﺪﮔﺎن در زردهی ﺗﺨﻢ ﺧﻮد اﯾﻤﻮﻧﻮﮔﻠﻮﺑﯿﻦ y (igy) ﮐﻪ ﻣﻌﺎدلigg ﭘﺴﺘﺎﻧﺪاران اﺳﺖ را ﻣﯽﺳﺎزﻧﺪ. در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﻪ ﺑﺮرﺳﯽ ﮐﯿﻔﯽ و ﮐﻤﯽ آﻧﺘﯽﺑﺎدیﻫﺎی اﺳﺘﺨﺮاج ﺷﺪه از زردهی ﺗﺨﻢ ﺑﺮﺧﯽ از ﭘﺮﻧﺪﮔﺎن اﻫﻠﯽ و زﯾﻨﺘﯽ از ﺟﻨﺒﻪﻫﺎی ﻣﺨﺘﻠﻒ ﭘﺮداﺧﺘﻪ ﺷﺪه اﺳﺖ. ﻣﺤﺘﻮای ﭼﺮﺑﯽ زرده ﺗﺨﻢ ﺷﺶ ﮔﻮﻧﻪ ﻣﺨﺘﻠﻒ از ﭘﺮﻧﺪﮔﺎن اﻫﻠﯽ و زﯾﻨﺘﯽ ﭘﺲ از ﺿﺪ ﻋﻔﻮﻧﯽ و اﻧﺪازهﮔﯿﺮی ﻣﯿﺎﻧﮕﯿﻦ وزﻧﯽ و ﻣﯿﺰان زرده، ﺟﺪا ﮔﺮدﯾﺪ. ﻣﯿﺰان ﺳﺮم ﺑﻪدﺳﺖ آﻣﺪه ﺛﺒﺖ و رﺳﻮب آﻧﺘﯽﺑﺎدی در دو ﻣﺮﺣﻠﻪ ﻣﺘﻮاﻟﯽ ﺑﺎ ﺳﻮﻟﻔﺎت آﻣﻮﻧﯿﻮم ﺻﻮرت ﮔﺮﻓﺖ و ﭘﺲ از دﯾﺎﻟﯿﺰ، آﻧﺘﯽ ﺑﺎدی اﺳﺘﺨﺮاج ﮔﺮدﯾﺪ. در ﻣﺮﺣﻠﻪ ﻧﻬﺎﯾﯽ، od ﻣﻘﺪار ﮐﻤّﯽ آﻧﺘﯽﺑﺎدی، ﺑﺮای ﻫﺮ ﮐﺪام از ﻧﻤﻮﻧﻪﻫﺎ ﺑﻪ ﻃﻮر ﺟﺪاﮔﺎﻧﻪ، در ﻃﻮل ﻣﻮجﻫﺎی ﻣﺘﻔﺎوت ﺑﺮﺣﺴﺐ ml/µg ﻣﺤﺎﺳﺒﻪ و ﺗﺠﺰﯾﻪ و ﺗﺤﻠﯿﻞ اﻧﺠﺎم ﮔﺮﻓﺖ. ﻣﯿﺎﻧﮕﯿﻦ وزﻧﯽ ﺗﺨﻢ ﻏﺎز، اردک، ﻣﺮغ ﺑﻮﻣﯽ، ﮐﺒﻮﺗﺮ، ﺑﻠﺪرﭼﯿﻦ و ﻣﺮغ ﻋﺸﻖ ﺑﻪ ﺗﺮﺗﯿﺐ: 12/4 ،19/5 ،57/4 ،62/7 ،145/5 و 6/1 ﮔﺮم و ﻧﺴﺒﺖ وزن زرده ﺑﻪ وزن ﺗﺨﻢ 0/31 ،0/32، 0/21 ،0/16 ،0/23 و 0/21 و ﻣﻘﺪار ﺳﺮم ﺑﻪ دﺳﺖ آﻣﺪه ﺑﻪ ﺗﺮﺗﯿﺐ ﺑﺮاﺑﺮ ﺑﺎ 12/5 ،52، 3 ،3/75 ،15 و 1/5 ﻣﯿﻠﯽﻟﯿﺘﺮ ﮔﺰارش ﺷﺪ. ﻧﺘﺎﯾﺞ ﻏﻠﻈﺖ آﻧﺘﯽﺑﺎدی اﺳﺘﺤﺼﺎﻟﯽ از ﻫﺮ ﺗﺨﻢ ﻧﯿﺰ ﺑﻪ ﺗﺮﺗﯿﺐ ﻗﺒﻠﯽ60450 ، 10631/25، 5203 ،12026 و 2535 ﻣﯿﮑﺮوﮔﺮم در ﻣﯿﻠﯽﻟﯿﺘﺮ در od280 ﺑﻪدﺳﺖ آﻣﺪ. ﻧﺘﺎﯾﺞ ﺣﺎﮐﯽ از ﺑﯿﺸﺘﺮﯾﻦ آﻧﺘﯽﺑﺎدی اﺳﺘﺤﺼﺎﻟﯽ از ﻏﺎز و ﺳﭙﺲ ﻣﺮغ ﺑﻮﻣﯽ و ﻫﻤﭽﻨﯿﻦ اﻣﮑﺎن اﺳﺘﻔﺎده ﻣﻨﺎﺳﺐ از ﺑﻠﺪرﭼﯿﻦ ﺑﻪ ﻋﻨﻮان ﺟﺎﯾﮕﺰﯾﻦ ﻣﺮغ ﺑﻮد.

comparative quantities and qualities study on avian igy antibody obtained from egg yolks of domestic and ornamental birds

background and aim: the birds produce igy immunoglobulin in their egg yolks, which is equivalent to the mammalian igg. in this study, we investigated the quality and quantity of antibody in egg yolks of some domestic and ornamental birds.methodseggs of six different species of domestic and ornamental birds after disinfection and weighing of the yolk content were examined and the content of yolk fat was separated. the serum level was recorded and two consecutive levels of antibody precipitation with ammonium sulfate were followed and after dialysis, the antibody was extracted. at the final stage, the od value were calculated and analyzed for each samples, separately.results: estimated weight average of geese, ducks, native chicks, pigeons, quail and chicken eggs were 145.5, 62.7, 57.4, 19.5, 12.4 and g 1.6, and the weight percentage of yolk/ egg were 0.32, 0.31, 0.23, 0.16, 0.21 and 0.21, respectively. the serum levels were observed as 52 ml, 12.5 ml, 15 ml, 3.75 ml, 3 ml, 5.1 ml, respectively. the results of the concentration of antibody to each egg were 60450 μg / ml, 10631.25 μg / ml, 12026 μg / ml, 5203 μg / ml and 2535 μg / ml in od280, respectively.conclusions: the results showed that the highest antibody production is related to geese and in next turn is for native chicken. also, the use of quail egg as chicken egg alternative could be advisable.