differential hepatic gene regulation in melanoma: combined exercise and anti-inflammatory supplementation selectively lowers cxcl2 but not bfgf2

Hepatic gene expression of inflammatory and growth factors such as il-8 and bfgf2 may be modulated in melanoma metastasis. non-pharmacological interventions like exercise and anti-inflammatory supplements represent potential complementary strategies for modification. this study aimed to investigate the effects of aerobic exercise, pineapple extract supplementation, and their combination on the hepatic expression of cxcl2/il-8 homolog and bfgf2 genes in a murine melanoma model. melanoma-bearing mice were allocated into four groups (n=5 per group): control, aerobic exercise, pineapple extract supplement, and aerobic exercise+pineapple extract. after the intervention period, liver tissue was analyzed for cxcl2/il-8 homolog and bfgf2 gene expression via one-way anova and tukey hsd test. pearson correlation assessed the relationship between the two genes. a significant difference was observed in cxcl2/il-8 homolog gene expression between groups (f=4.211, p=0.0239). post hoc analysis revealed that only the combined aerobic exercise + pineapple extract group showed a significant decrease in hepatic cxcl2/il-8 homolog compared to the cancer control group (p=0.0251). in contrast, no significant difference was found in bfgf2 gene expression across groups (f=1.425, p=0.2745). correlation analysis indicated a significant negative relationship between cxcl2/il-8 homolog and bfgf2 exclusively in the cancer control group (r=-0.948, p=0.013). the combination of aerobic exercise and pineapple extract supplementation significantly reduces hepatic cxcl2/il-8 homolog expression in melanoma-bearing mice, suggesting a potential synergistic effect in modulating the hepatic inflammatory microenvironment. the distinct lack of effect on bfgf2 and the specific negative correlation in controls highlight pathway-selective responses.