the combined effects of resistance training and pineapple extract on intratumoral nf-κb, lin28b, and systemic tnf-α in a murine melanoma model

This study investigated the effects of resistance training and pineapple extract consumption on intratumoral nf-κb and lin28b gene expression and serum tnf-α levels in a murine c57 melanoma model. twenty c57bl/6 mice were allocated into four groups (n=5/group): melanoma tumor control (mt), mt with resistance training (mt+rt), mt with pineapple extract (mt+pj), and mt with combined intervention (mt+rt+pj). the rt protocol and pj administration (300 mg/kg/day via gavage) were conducted for six weeks’ post-tumor induction. serum tnf-α was quantified by elisa, and tumor gene expression of nf-κb and lin28b was analyzed via rt-pcr. data were analyzed using one-way anova followed by tukey's post hoc test. all three intervention groups exhibited a significant downregulation of nf-κb and lin28b gene expression in tumor tissue compared to the mt control group (p<0.05). conversely, serum tnf-α levels were significantly elevated in the intervention groups relative to the control (p<0.05). resistance training and pineapple extract consumption, both individually and in combination, significantly modulated pro-tumorigenic pathways by suppressing intratumoral nf-κb and lin28b expression, despite an observed increase in systemic tnf-α.