isolation and characterization of neoduline from the rhizome of dolichos pachyrhizus harm

Dolichos is a genus of twining plants found in both the northern and southern hemispheres, with the most frequently grown and used species being dolichos biflorus and dolichos lablab. antimicrobial, antioxidant, anticancer, anti-schistosomal, and anti-inflammatory properties have been reported in various parts of the dolichos species, including the leaves, roots, bark, and stem. the rhizome of dolichos pachyrhizus was cut, air-dried at ambient temperature, and macerated with n-hexane, dichloromethane, ethyl acetate, and methanol for 72 hours each using a conventional maceration procedure, with regular shaking at intervals. the mixture was decanted, filtered, and concentrated at 40 °c using a rotavapor to yield crude n-hexane, dichloromethane, ethyl acetate, and methanol extracts (r110). to obtain the pure isolate, a methanol crude extract of the rhizome of dolichos pachyrhizus (d. pachyrhizus) was chromatographed on a silica gel column using various eluents. the preliminary phytochemical investigation of the methanolic rhizome extract of dolichos pachyrhizus revealed the presence of seven (7) different phytochemicals, including; - alkaloids, flavonoids, glycosides, phenols, tannins, saponins and steroids. the structure of the pure isolate was successfully identified, characterized and confirmed using spectroscopic techniques such as; - fourier transform spectroscopy (ft-ir), gas chromatography-mass spectrometry (gc-ms) and nuclear magnetic resonance (nmr) be neoduline.