molecular identification of pasteurella spp. in poultry serum and liver samples by pcr in the central region of iraq (babylon)

Pasteurellosis is a zoonotic disease that affects a variety of animal species. it is caused by bacteria belonging to the genus pasteurella, which can result in economic losses for the poultry and livestock industries.  the most prevalent modes of transmission of the bacterium to humans are through bites or scratches inflicted by infected animals and contact with the mucosal membranes or blood of said animals. humans can contract pasteurellosis by being bitten or scratched by infected animals, or through contact with their mucus or blood. a cross-sectional study was conducted in mid-2023. two hundred sixty serum samples and 175 liver tissue samples were collected from ten broiler farms (age range: 30–42 days) in the central regions of iraq for during the year 2023. the samples underwent dna extraction using a dna extraction kit from pooya gen azma, iran. identification up to genus pasteurella spp. was done using the 16s rrna gene. the primers were designed using amplifix version 1.5.4 software. in this study, the pcr amplification was done on serum and liver dna samples. the results indicated that 63 of the serum samples (i.e., 24.23%, [95% ci: 19.42 - 29.79]) and 26 of the liver samples (i.e., [14.80%, 95% ci: 10.35- 20.90]) were positive for the genus pasteurella. the findings of this study help to elucidate the existing gaps in knowledge regarding pasteurella spp. such research will undoubtedly contribute to the greater overall protection of human health.